BubR1 Antibody (8G1)

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Product Details

Summary
Product Discontinued
View other related BubR1 Primary Antibodies

Order Details


    • Catalog Number
      NB100-1685
    • Availability
      Product Discontinued

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BubR1 Antibody (8G1) Summary

Immunogen
N-terminal tagged fusion recombinant fragment, corresponding to amino acids 1-350 of Human BubR1.
Localization
Cytoplasmic
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
BUB1B
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:200-1:1000
  • ELISA 1:5000-1:20000
  • Immunocytochemistry/Immunofluorescence 1:200-1:1000
  • Immunoprecipitation 1:100
Application Notes
This protein A purified antibody has been tested for use in immunoprecipitation, Immunofluorescence staining and western blot and is capable of detecting endogenous protein. Expect a predominant band at ~ 120 kDa corresponding - full-length protein by western blotting in the appropriate cell lysate or extract. Higher MW bands may be seen that may be Due to hyper-phosphorylation of the protein. The use of HeLa whole cell lysates is recommended as a positive control. For IF microscopy use cells grown on cover slips fixed with 3.5% paraformaldehyde in PBS at pH 6.8. Permeabilize fixed cells with 0.2% Triton X-100 in 25 mM Tris Cl, pH 7.4 containing 0.1% BSA.
Positive Control
BubR1 Lysate (NBL1-08057)
Publications
Read Publication using
NB100-1685 in the following applications:

  • IP
    1 publication

Reactivity Notes

This Protein A purified antibody is directed against human BUBR1 Kinase protein. The product was purified from tissue culture supernatant by chromatography. This antibody has only been tested on human cells. Reactivity against homologues from other sources is not known.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Purity
Protein A purified

Alternate Names for BubR1 Antibody (8G1)

  • Bub1A
  • BUBR1budding uninhibited by benzimidazoles 1 (yeast homolog), beta
  • budding uninhibited by benzimidazoles 1 homolog beta (yeast)
  • EC 2.7.11.1
  • hBUBR1
  • MAD3/BUB1-related protein kinase
  • MAD3LBUB1beta
  • Mitotic checkpoint kinase MAD3L
  • Protein SSK1
  • SSK1mitotic checkpoint serine/threonine-protein kinase BUB1 beta

Background

BUBR1 Kinase (also called Mitotic checkpoint serine/threonine-protein kinase, BUB1 beta, MAD3/BUB1-related protein kinase, Mitotic checkpoint kinase MAD3L and SSK1) is a probable component of the mitotic checkpoint that delays anaphase until all chromosomes are properly attached to the mitotic spindle. This protein can interact with BUB3, CENP-F, CENP-E and mitosin. BUBR1 Kinase is found within the cytoplasm in interphase cells, but when bound to BUB3 or CENP-E, it can be localized to nuclear kinetochores. This protein is highly expressed in thymus followed by spleen. Defects in BUB1B are associated with tumor formation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for BubR1 Antibody (NB100-1685)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: IP.


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Product General Protocols

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Video Protocols

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ICC/IF Video Protocol

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Positive Control Lysate(s)

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Bioinformatics

Gene Symbol BUB1B
Entrez