BubR1 Antibody (11G2)

Images

 
Western Blot: BubR1 Antibody (11G2) [NBP1-47686] Analysis of extracts (35ug) from 9 different cell lines by using anti-BubR1 monoclonal antibody.
Immunocytochemistry/ Immunofluorescence: BubR1 Antibody (11G2) [NBP1-47686] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY BubR1.
Immunohistochemistry-Paraffin: BubR1 Antibody (11G2) [NBP1-47686] - Staining of paraffin-embedded Carcinoma of thyroid tissue using anti-BubR1 mouse monoclonal antibody.
Western Blot: BubR1 Antibody (11G2) [NBP1-47686] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BubR1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates ...read more
Immunohistochemistry-Paraffin: BubR1 Antibody (11G2) [NBP1-47686] - Staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-BubR1 mouse monoclonal antibody.

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP1-47686
    • Availability
      Product Discontinued

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BubR1 Antibody (11G2) Summary

Immunogen
Protein expressed in 293T cell transfected with human BUB1B expression vector
Specificity
This antibody is specific for Homo sapiens budding uninhibited by benzimidazoles 1 homolog beta (yeast) (BUB1B).
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
BUB1B
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50

Reactivity Notes

Human, Primate

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for BubR1 Antibody (11G2)

  • Bub1A
  • BUBR1budding uninhibited by benzimidazoles 1 (yeast homolog), beta
  • budding uninhibited by benzimidazoles 1 homolog beta (yeast)
  • EC 2.7.11.1
  • hBUBR1
  • MAD3/BUB1-related protein kinase
  • MAD3LBUB1beta
  • Mitotic checkpoint kinase MAD3L
  • Protein SSK1
  • SSK1mitotic checkpoint serine/threonine-protein kinase BUB1 beta

Background

This gene encodes a kinase involved in spindle checkpoint function. The protein has been localized to the kinetochore and plays a role in the inhibition of the anaphase-promoting complex/cyclosome(APC/C), delaying the onset of anaphase and ensuring proper chromosome segregation. Impaired spindle checkpoint function has been found in many forms of cancer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Bioinformatics

Gene Symbol BUB1B
Entrez