Bub3 Antibody

Images

 
Western Blot: Bub3 Antibody [NBP1-19630] - extracts from HeLa cells.
Immunohistochemistry-Paraffin: Bub3 Antibody [NBP1-19630] - Paraffin-embedded human brain tissue.

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP1-19630
    • Availability
      Product Discontinued

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Bub3 Antibody Summary

Immunogen
Synthetic peptide, corresponding to amino acids 251-300 of Human BUB3.
Clonality
Polyclonal
Host
Rabbit
Gene
BUB3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50-1:200
Application Notes
Western blot (WB) analysis of Bub3 (H274) pAb in extracts from HeLa cells.
Immunohistochemistry (IHC) analyzes of Bub3 (H274) pAb in paraffin-embedded human brain tissue.
Theoretical MW
40 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human, Mouse, Rat

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2)
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Bub3 Antibody

  • BUB3 (budding uninhibited by benzimidazoles 3, yeast) homolog
  • BUB3 budding uninhibited by benzimidazoles 3 homolog
  • Bub3
  • BUB3L
  • budding uninhibited by benomyl
  • budding uninhibited by benzimidazoles 3 homolog (yeast)
  • hBUB3
  • mitotic checkpoint component
  • mitotic checkpoint protein BUB3

Background

The mitotic checkpoint protein Bub3 appears to be essential during normal mitotic progression, preventing premature sister chromatid separation, missegreation and aneuploidy. Bub3 is also important during G2 and early mitosis stages, promoting normal mitotic entry. Loss of Bub3 function causes slower progression through prophase, apparently due to mitotic cyclins A and B not being accumulated.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Bub3 Antibody (NBP1-19630) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for Bub3 Antibody (NBP1-19630)

Discover related pathways, diseases and genes to Bub3 Antibody (NBP1-19630). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Research Areas for Bub3 Antibody (NBP1-19630)

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Contact Information

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Calculators

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Bioinformatics

Gene Symbol BUB3
Entrez