Bub3 Antibody


Western Blot: Bub3 Antibody [NB100-572] - Samples: Whole cell lysate (50 ug) from HeLa cells treated with Nocodazole (+) or mock treated (-). Antibody: Affinity purified rabbit anti-Bub3 antibody used for WB at 0.066 ...read more
Immunohistochemistry-Paraffin: Bub3 Antibody [NB100-572] - Human prostate carcinoma. Antibody used at a dilution of 1:1000 (1ug/ml).
Western Blot: Bub3 Antibody [NB100-572] - Whole cell lysate (50 ug) from HeLa cells treated with Nocodazole (+) or mock treated (-). Antibody used at 0.1 ug/ml.

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Bub3 Antibody Summary

A synthetic peptide which maps to a region between residues 1 and 50 of human BUB3 using the numbering given in Swiss-Prot entry O43684 (GeneID 9184).
Immunogen affinity purified
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  • Western Blot 1:5000-1:15000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
Application Notes
Optimal working dilutions should be determined experimentally by the investigator. Prepare working dilution immediately before use. Suggested starting dilutions are as follows. Western Blot: 1:5,000 - 1:15,000 Immunoprecipitation: not tested Histo/Cytochemistry: not tested *In some cases, the antibody may be diluted further than indicated.
Positive Control
Bub3 Lysate (NBL1-08058)
HeLa Lysate (NB800-PC1)

Packaging, Storage & Formulations

Store at 4C. Do not freeze.
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
0.09% Sodium Azide
1.0 mg/ml
Immunogen affinity purified

Alternate Names for Bub3 Antibody

  • BUB3 (budding uninhibited by benzimidazoles 3, yeast) homolog
  • BUB3 budding uninhibited by benzimidazoles 3 homolog
  • Bub3
  • BUB3L
  • budding uninhibited by benomyl
  • budding uninhibited by benzimidazoles 3 homolog (yeast)
  • hBUB3
  • mitotic checkpoint component
  • mitotic checkpoint protein BUB3


The spindle checkpoint ensures the fidelity of chromosome segregation in mitosis and meiosis. In response to defects in the mitotic apparatus, it blocks the activity of the anaphase-promoting complex, a large ubiquitin ligase required for chromosome segregation. Recent studies indicate that the spindle checkpoint monitors both the attachment of chromosomes to the mitotic spindle and the tension across the sister chromatid generated by microtubules. Upon checkpoint activation, checkpoint protein complexes containing BubR1(Mad3), Bub3, Mad2 and Cdc20 directly bind to the anaphase-promoting complex and inhibit its ligase activity. Therefore, the checkpoint proteins form a complex intracellular signalling network to inhibit the anaphase-promoting complex.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Positive Control Lysate(s)

Secondary Antibodies


Isotype Controls

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Gene Symbol BUB3