Bub1 Antibody

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Immunohistochemistry-Paraffin: Bub1 Antibody [NBP1-40009] - Analysis of anti-BUB1 antibody with human small intestine at concentration 5 ug/ml.

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP1-40009
    • Availability
      Product Discontinued

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Bub1 Antibody Summary

Immunogen
Synthetic peptide from human BUB1.
Specificity
a 19 amino acid peptide from near the amino terminus of human Bub1
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
BUB1
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 4 ug/ml
  • Immunohistochemistry-Paraffin 5 ug/ml

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Bub1 Antibody

  • BUB1 budding uninhibited by benzimidazoles 1 homolog
  • Bub1
  • BUB1Amitotic checkpoint serine/threonine-protein kinase BUB1
  • BUB1L
  • budding uninhibited by benzimidazoles 1 (yeast homolog)
  • budding uninhibited by benzimidazoles 1 homolog (yeast)
  • EC 2.7.11.1
  • hBUB1mitotic spindle checkpoint kinase
  • putative serine/threonine-protein kinase

Background

Chromosome segregation and mitotic checkpoints are regulated by various proteins among them are BUB1 and BUBR1/BUB1B (Budding Uninhibited by Benzimidazoles 1). During mitosis both BUB1 and BUBR1 proteins are localized to kinetochores and inhibit the anaphase stage until all chromosomes are correctly attached to the spindle. These proteins were found due to their homology to Bub1 protein from yeast. Human BUBR1 contains CD1 and CD2 domains that are found in human, mouse and yeast BUB1.3-5 The human and mouse BUBR1 proteins are 29% identical in these regions. The kinetochore localization and Bub3 binding is found in the CD1 region while the kinase domain is found in the CD2 domain. While both human and mouse BUB1 contain a nuclear localization site between CD1 and CD2 regions, the yeast protein does not contain this site. BUB1 is expressed in many tissues with the highest expression in testis and thymus, lower expression in colon and small intestine and very low expression in spleen and ovary. BUB1 expression is in correlation with the expression of MAD2, another human spindle assembly checkpoint protein.3-5 BUBR1 is mainly expressed in testis, thymus and bone marrow while in differentiated tissues such as brain, heart and muscle the expression is low.5

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol BUB1
Entrez
Uniprot