BTLA/CD272 Antibody

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Western Blot: BTLA/CD272 Antibody [NBP1-06981] - (1ug/ml) Analysis of Mouse Spleen lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

BTLA/CD272 Antibody Summary

Immunogen
Peptide with sequence KEAPTEYASICVRS corresponding to C-Terminus according to NP_001078826.1.
Epitope
C Terminus
Specificity
This antibody is expected to recognize both reported isoforms (NP_861445.3; NP_001078826.1).
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
BTLA
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 - 3 ug/ml
  • Peptide ELISA Detection limit 1:4000
Application Notes
WB: Approx. 35 kDa band observed in mouse spleen lysates (calculated MW of 32.8 kDa band according to human NP_861445.3 and of 34.2 kDa band according to mouse NP_001032808.2).
Publications
Read Publication using NBP1-06981.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Human, Rat.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for BTLA/CD272 Antibody

  • B and T lymphocyte associated
  • B and T lymphocyte attenuator
  • B- and T-lymphocyte attenuator
  • B- and T-lymphocyte-associated protein
  • BTLA
  • BTLA1
  • CD272 antigen
  • CD272
  • FLJ16065
  • MGC129743

Background

BTLA expression is induced during activation of T cells, and BTLA remains expressed on Th1 cells but not Th2 cells. Like PD1 (MIM 600244) and CTLA4 (MIM 123890), BTLA interacts with a B7 homolog, B7H4.[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for BTLA/CD272 Antibody (NBP1-06981)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol BTLA
Entrez
Uniprot