Antibody FITC-BtBd7 is ideal for WB and ELISA applications, other applications have not been tested. Immunohistochemistry-Paraffin was reported in scientific literature. The species cross reactivity for these antibodies have not been examined fully. The dilutions for this antibody is for reference only, investigators are expected to determine the optimal conditions for specific assay. WB: > 1:500; IMM & i.p pull-down assays: 1:200; IHC 1:200. ELISA <1:10,000. Application of this antibody in protocols not listed here does not necessarily exclude its use in such procedures.
Read Publications using NBP1-62182 in the following applications:
Mouse reactivity reported in scientific literature (PMID: 11231300)
Packaging, Storage & Formulations
Store at -20C. Avoid freeze-thaw cycles.
Tris/Glycine buffer pH 7.5-7.9, stabilizing protein, and glycerol
0.02% Sodium Azide
Immunogen affinity purified
Alternate Names for BTBD7 Antibody [FITC]
BTB (POZ) domain containing 7
FUP1BTB/POZ domain-containing protein 7
Cancer cells especially human hepatocellular carcinoma (HCC) differentially express many genes as determined by cDNA microarrays. One of these genes is Btbd7 whose function was not known. When transfected in NIH3T3 cells this gene exhibited enhanced cell proliferation activity by MTT assay. The enhanced tumorigencity was blocked by anti-sense RNA experiments (1). Recently it has been shown that Btbd7 regulate epithelial cell dynamics and branching morphogenesis. The organ development during embryogenesis requires extensive branching of epithelia via formation of cleft and buds. These branches are developed by local outgrowth or by formation of cleft that further divide epithelia in to buds. The bud or tubule extension is controlled and regulated by various growth factors. These branching finally led to formation of various organs. Cellular matrix protein fibronectin is required for salivary, lung and kidney branching (3). The edges of fibronectin translocates inwards as cleft accompanied by loss of E-cadherin in cells adjacent to fibronectin. The Btbd7 activity was noticed at cell cleft forming sites (2). The cleft formation involves buds delineation of buds by conversion of epithelial cell-cell adhesion to cell-matrix adhesion. Btbd7 provides a mechanistic link between the extracellular matrix and cleft propagation via its highly discrete expression that control epithelial cell motility by interaction with other cellular architectural and scaffolding proteins like Snail2 (slug), E-cadherin (4). The Btbd7 antibodies were generated using peptide corresponding to C-terminal domain of the human Btbd7 gene. The Btbd7 antibodies are affinity purified over immobilized antigen based affinity chromatography, and the purified immunoglobulins are stabilized in antibody stabilization buffer.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
FAQs for BTBD7 Antibody (NBP1-62182). (Showing 1 - 1 of 1 FAQs).
I read on your website that NBP1-62182 has been mentioned in at least 3 Publications, and one of them was Onodera et al. 2010, Science 329(5991):562-5. However, I cannot find any mentioning of this antibody, nor can I find a FITC-image. Also, the first publication mentioned (Dressler GR 2006) is only a review, and the second one doesn't seem to mention it either. Can you please enlighten me, as I am looking for an antibody that is suitable for IHC on mouse tissue?
NBP1-62182 is the FITC conjugated version of NBP1-49652 which is why you are not seeing the FITC image. However, neither of these antibodies has been validated for IHC. I would recommend NBP2-14364 as the immunogen shares 98% similarity to mouse and is predicted to cross-react. While we can not guarantee this, if you would like to test this antibody in an untested species and share your results with us, then I can recommend our Innovators Reward Program. Under this program, you can complete an online review with image, detailing your positive or negative results and in return, you receive a discount voucher for 100% of the purchase price of the reviewed product.
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