Bromodeoxyuridine/BrdU Antibody

Images

 
Immunocytochemistry/ Immunofluorescence: Bromodeoxyuridine/BrdU Antibody [NB500-235] - IF analysis of BrdU in Proteinase K pretreated mouse lacrimal gland. Image courtesy of anonymous customer review.
Immunoprecipitation: Bromodeoxyuridine/BrdU Antibody [NB500-235] - Lysate was immunoprecipitated with anti-brdu antibody (NB500-235), 5-methyl cytosine (5-Me), or a no antibody control (No A). An ELISA was then ...read more

Product Details

Summary
Reactivity Mu, AllSpecies Glossary
Applications ICC/IF, IHC, IHC-Fr, IHC-P, In vitro, IP
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Concentration
3.2 mg/ml

Order Details

Bromodeoxyuridine/BrdU Antibody Summary

Immunogen
Bromodeoxyuridine (BrdU) coupled to keyhole limpet hemocyanin (KLH).
Marker
Proliferation Marker
Specificity
BrdU
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 1:100-1:200
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 10 ug/ml
  • Immunohistochemistry-Paraffin 10 ug/ml
  • Immunoprecipitation 25-100 ug/ml
  • In vitro assay
Application Notes
This BrdU antibody is useful for ICC/IF, IHC-frozen, IHC-paraffin, and IP.
Control
Donkey Whole Serum (NBP1-50088)
Reviewed Applications
Read 2 Reviews rated 5
using
NB500-235 in the following applications:

Publications
Read Publications using
NB500-235 in the following applications:

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
No Preservative
Concentration
3.2 mg/ml
Purity
Protein G purified

Alternate Names for Bromodeoxyuridine/BrdU Antibody

  • 5-bromo-2-deoxyuridine
  • BrdU
  • Bromodeoxyuridine
  • BUdr

Background

BrdU (Bromodeoxyuridine) is a synthetic nucleoside that is incorporated into actively replicating cells' DNA. In many studies, BrdU incorporation is used as a marker for the proliferation of cells. This technique is particularly valuable in detecting proliferation in tissues that are not easily accessible to other assay types, like the brain. It is also commonly used to detect developmental progress in embryogenesis. Detection of the concentration and pattern of BrdU incorporation in tissues can give clues as to the pattern, rate, and progression of cell proliferation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Bromodeoxyuridine/BrdU Antibody (NB500-235)(15)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Guinea Pig.

We have publications tested in 4 applications: ICC/IF, IHC, IHC-Fr, In vitro.


Filter By Application
ICC/IF
(4)
IHC
(4)
IHC-Fr
(1)
In vitro
(1)
All Applications
Filter By Species
Human
(1)
Mouse
(1)
Rat
(1)
Guinea Pig
(1)
All Species
Showing Publications 1 - 10 of 15. Show All 15 Publications.
Publications using NB500-235 Applications Species
Xu H, Zhang L, Chen W et al. Inhibitory effect of caveolin-1 in vascular endothelial cells, pericytes and smooth muscle cells Oncotarget. 2017 Sep 29 [PMID: 29100301] (In vitro, Human) In vitro Human
Gilbert ME, Goodman JH, Gomez J et al. Adult hippocampal neurogenesis is impaired by transient and moderate developmental thyroid hormone disruption Neurotoxicology Dec 31 2016 [PMID: 28048979] (Rat) Rat
Lin CY, Zu CH, Yang CC et al. IL-1beta-induced mesenchymal stem cell migration involves MLCK activation via PKC signaling. Cell Transplantation 2015 [PMID: 25333338] (IHC-Fr)

Details:
Using the DyLight 488 conjugated version of NB500-235, catalog number NB500-235G.
IHC-Fr
Puzzo D, Bizzoca A, Privitera L et al. F3/Contactin promotes hippocampal neurogenesis, synaptic plasticity, and memory in adult mice. Hippocampus. 2013 Aug 12 [PMID: 23939883] (IHC, ICC/IF) IHC, ICC/IF
Tveden-Nyborg P, Vogt L et al. Maternal Vitamin C Deficiency during Pregnancy Persistently Impairs Hippocampal Neurogenesis in Offspring of Guinea Pigs PLoS One 2012 [PMID: 23119033] (IHC, ICC/IF, Guinea Pig) IHC, ICC/IF Guinea Pig
Criswell KA, Cook JC, Morse D et al. Pregabalin Induces Hepatic Hypoxia and Increases EndothelialCell Proliferation in Mice, a Process Inhibited by DietaryVitamin E Supplementation Toxicol Sci 2012 Jul [PMID: 22539613] (IHC, Mouse) IHC Mouse
You S, Tariq A, Kublin CL, Zoukhri D. Detection of BrdU-label retaining cells in the lacrimal gland: implications for tissue repair. Cell and Tissue Research. 2011 Nov 19. [PMID: 22101331]
Tulloch I, Afanador L, Mexhitaj I et al. A single high dose of methamphetamine induces apoptotic and necrotic striatal cell loss lasting up to 3 months in mice. Neuroscience. 2011 Jul 16. [PMID: 21771641]
Zhang J, Zhang Y, Dutta DJ et al. Proapoptotic and Antiapoptotic Actions of Stat1 versus Stat3 Underlie Neuroprotective and Immunoregulatory Functions of IL-11. J Immunol;187(3):1129-41. 2011 Aug 1. [PMID: 21709156]
Kent D Dunlap, Anna C Silva Michael Chung. Environmental complexity, seasonality and brain cell proliferation in a weakly electric fish, Brachyhypopomus gauderio. J exp Biol; 214, 794-805. 2011 [PMID: 21307066] (IHC) IHC
Show All 15 Publications.

Reviews for Bromodeoxyuridine/BrdU Antibody (NB500-235) (2) 52

Average Rating: 5
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Mouse.

Reviews using NB500-235:
Filter by Applications
IF
(1)
IHC-Fr
(1)
All Applications
Filter by Species
Mouse
(2)
All Species
Images Ratings Applications Species Date Details
Immunofluorescence Bromodeoxyuridine/BrdU NB500-235
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reviewed by:
Claire Kublin
IF Mouse 03/15/2011
View

Summary

ApplicationImmunofluorescence
Sample TestedMouse Lacrimal Gland
SpeciesMouse
CommentsWe used this BrdU antibody on lacrimal gland tissue from a mouse. We used the antibody at a concentration of 1:100 or 1:200 and a proteinase K treatment was applied.

Blocking

Blocking DetailsBlocking Buffer: 10% Normal Donkey Serum in PBS, Blocking Time: 1 hour, Blocking Temp: 25 degrees Celsius

Primary Anitbody

Dilution RatioPrimary Ab Incubation Dilution Buffer: 1% NDS in PBS, Dilution Ratio: 1:100, Incubation Time: overnight, Incubation Temp: 4C

Secondary Antibody

Secondary DescriptionSecondary Ab: FITC-Donkey anti-Sheep from Jackson Labs, Secondary Ab Dilution: 1:100

Details

Detection NotesDetection Visualization Method: Fluorescence at 488
Fixation DetailsSample Preparation: 4% Formalin

Comments

CommentsWe used this BrdU antibody on lacrimal gland tissue from a mouse. We used the antibody at a concentration of 1:100 or 1:200 and a proteinase K treatment was applied.
 
reviewed by:
Anonymous
IHC-Fr Mouse 05/27/2009
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample Testedmouse brain tissue section (dorsal striatum)
SpeciesMouse
LotP13083008
Commentsfantastic product

Blocking

Blocking DetailsBlocking Buffer: Donkey Serum or Goat serum, Blocking Time: 1 hour, Blocking Temp: room temperature

Primary Anitbody

Dilution RatioDilution Ratio:1/500, Incubation Dilution Buffer:1% triton X in PBS and Donkey or goat serum,Incubation Time:24 hrs, Temp: 4C

Secondary Antibody

Secondary DescriptionSecondary Ab: FITC donkey anti-sheep or goat anti-sheep, Secondary Ab Dilution: 1/200
Secondary Manufacturer Cat#NB740

Comments

Commentsfantastic product

Product General Protocols

View specific protocols for Bromodeoxyuridine/BrdU Antibody (NB500-235): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

ICC/IF Video Protocol

FAQs for Bromodeoxyuridine/BrdU Antibody (NB500-235) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional Array Products

Research Areas for Bromodeoxyuridine/BrdU Antibody (NB500-235)

Find related products by research area.

Blogs on Bromodeoxyuridine/BrdU.

Developmental regulator Daam2 promotes glial cell tumors by degrading Von Hippel-Lindau protein
By Jamshed Arslan Pharm.D. Glioblastoma is an aggressive type of cancer that forms from the star-shaped glial cells of the central nervous system, called astrocytes. Intriguingly, several genes linked to glioblasto...  Read full blog post.

BrDU (Bromodeoxyuridine)
The thymidine synthetic nucleoside analogue bromodeoxyuridine (BrdU) has a long, colorful history of repeated use in molecular and cytokinetic studies, as detailed in reviews by Vanderlaan and Dolbeare (1,2).  Because BrDU is only incorporated in...  Read full blog post.

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Recent Reviews

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Claire Kublin
03/15/2011
Application: IF
Species: Mouse

 
Anonymous
05/27/2009
Application: IHC-Fr
Species: Mouse