Bromodeoxyuridine/BrdU Antibody (IIb5)

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Product Details

Summary
Reactivity RtSpecies Glossary
Applications Flow, ICC/IF, IHC, IHC-Fr, IHC-P
Clone
IIb5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

Bromodeoxyuridine/BrdU Antibody (IIb5) Summary

Immunogen
BrdU coupled to BSA. In immunoassays this antibody reacts strongly with free or carrier-protein coupled BrdU but not with other nucleosides. In immuncytochemistry the antibody only recognizes BrdU in denaturated (single stranded) DNA. The BrdU antibody is
Marker
Proliferation Marker
Specificity
BrdU is a thymidine analogue and when offered to proliferating cells it is incropora­ted into reduplicating DNA. The antibody is specific for DNA in which BrdU has been incorporated. In immunoassays this antibody reacts strongly with free or carrier-protein coupled BrdU but not with other nucleosides. In immuncytochemistry the antibody only recognizes BrdU in denaturated (single stranded) DNA. The BrdU antibody is 100% cross reactive with Jodo-Deoxy-Uridine (IrdU). Therfore, IdU instead of BrdU can be used for in vivo studies.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Purity
Unpurified
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Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
Application Notes
Pulse labeling of dividing cells will allow the immunocytochemical identification of S-phase cells. BrdU incorporating can be analyzed in vitro cell cultures by adding BrdU to the tissue culture medium (10µM final conc.). Exposure periods as brief as 10The antibody can be used on frozen sections and on paraffin embedded tissue after protease digestion. Dilute antibody in 0.15 M phosphate buffered saline with 1% BSA and 1% Na-azide. Advised working dilution: 1:5 - 1:10. Optimal dilution should be tested

Reactivity Notes

Rat

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Ascites
Preservative
0.02% Sodium Azide
Purity
Unpurified

Alternate Names for Bromodeoxyuridine/BrdU Antibody (IIb5)

  • 5-bromo-2-deoxyuridine
  • BrdU
  • Bromodeoxyuridine
  • BUdr

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

FAQs for Bromodeoxyuridine/BrdU Antibody (NBP1-42260) (0)

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Secondary Antibodies

 

Isotype Controls

Additional Bromodeoxyuridine/BrdU Products

Bromodeoxyuridine/BrdU NBP1-42260

Blogs on Bromodeoxyuridine/BrdU.

BrDU (Bromodeoxyuridine)
The thymidine synthetic nucleoside analogue bromodeoxyuridine (BrdU) has a long, colorful history of repeated use in molecular and cytokinetic studies, as detailed in reviews by Vanderlaan and Dolbeare (1,2).  Because BrDU is only incorporated in...  Read full blog post.

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