Bromodeoxyuridine/BrdU Antibody (BU1/75 (ICR1)) [Biotin]

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Product Details

Summary
Reactivity Mu, NASpecies Glossary
Applications WB, ICC/IF, IHC, IHC-Fr, IHC-P
Clone
BU1/75 (ICR1)
Clonality
Monoclonal
Host
Rat
Conjugate
Biotin

Bromodeoxyuridine/BrdU Antibody (BU1/75 (ICR1)) [Biotin] Summary

Immunogen
Made to Chemical BrdU
Localization
Nuclear
Marker
Proliferation Marker
Specificity
This reacts with BrdU in single stranded DNA, BrdU attached to a protein carrier or free BrdU. It detects nucleated cells in S-Phase which have had BrdU incorporated into their DNA. Also reacts weakly with chlorodeoxyuridine, but does not cross react with thymidine or iododeoxyuridine.
Isotype
IgG2a
Clonality
Monoclonal
Host
Rat
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
Readout System
Reviewed Applications
Read 1 Review rated 4
using
NB500-169B in the following applications:

Publications
Read Publication using
NB500-169B in the following applications:

  • IHC
    1 publication

Reactivity Notes

Chemical.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Notes

This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for Bromodeoxyuridine/BrdU Antibody (BU1/75 (ICR1)) [Biotin]

  • 5-bromo-2-deoxyuridine
  • BrdU
  • Bromodeoxyuridine
  • BUdr

Background

The immunocytochemical detection of bromodeoxyuridine (BrdU) incorporated into DNA is a powerful tool to study the cytokinetics of normal and neoplastic cells. In vitro or in vivo labeling of cells with the thymidine analogue BrdU and the subsequent detection of incorporated BrdU with specific anti-BrdU monoclonal antibodies is an accurate and comprehensive method to quantitate the degree of DNA-synthesis. BrdU is incorporated into the newly synthezised DNA of the S-phase cells and can thus provide an estimate for the fraction of cells in S-phase. Also dynamic proliferative information (such as the S-phase transit rate and the potential doubling time) can be obtained, by means of bivariate BrdU/DNA flow cytometric analysis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Bromodeoxyuridine/BrdU Antibody (NB500-169B)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: IHC.


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IHC
(1)
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Mouse
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Review for Bromodeoxyuridine/BrdU Antibody (NB500-169B) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.
We have 1 review tested in 1 application: IHC.

Reviews using NB500-169B:
Filter by Applications
IHC
(1)
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Filter by Species
Mouse
(1)
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Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
IHC Mouse 12/30/2011
View

Summary

ApplicationImmunohistochemistry
Sample TestedMouse
SpeciesMouse

Blocking

Blocking DetailsBlocking Buffer: 10% NHS blocking

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:100, Primary Ab Incubation Time: overnight, Primary Ab Incubation Temp: 4 degrees Celsius

Details

Detection NotesDetection Visualization Method: DAB

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Bromodeoxyuridine/BrdU Antibody (NB500-169B) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

DyLight 488 Labeled NB500-169G
DyLight 550 Labeled NB500-169R
DyLight 650 Labeled NB500-169C
HRP Labeled NB500-169H

Additional Bromodeoxyuridine/BrdU Products

Bromodeoxyuridine/BrdU NB500-169B

Blogs on Bromodeoxyuridine/BrdU.

BrDU (Bromodeoxyuridine)
The thymidine synthetic nucleoside analogue bromodeoxyuridine (BrdU) has a long, colorful history of repeated use in molecular and cytokinetic studies, as detailed in reviews by Vanderlaan and Dolbeare (1,2).  Because BrDU is only incorporated in...  Read full blog post.

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Recent Reviews

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Anonymous
12/30/2011
Application: IHC
Species: Mouse