Brg1 Antibody

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Western Blot: Brg1 Antibody [NBP1-51230] - Extracts from K562 cells

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP1-51230
    • Availability
      Product Discontinued

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Brg1 Antibody Summary

Immunogen
Synthetic peptide, corresponding to amino acids 1600-1650 of Human SMARCA4.
Specificity
Brg-1 (R1614) pAb detects endogenous levels of Brg-1 protein.
Clonality
Polyclonal
Host
Rabbit
Gene
SMARCA4
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
Application Notes
Western blot (WB) analysis of Brg-1 (R1614) pAb in extracts from K562 cells
Theoretical MW
200 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Cross reacts with Human, Mouse and Rat.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (approx. pH 7.2)
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Brg1 Antibody

  • ATP-dependent helicase SMARCA4
  • BAF190
  • BAF190A
  • brahma protein-like 1
  • Brg1
  • BRG1-associated factor 190A
  • BRG1SWI/SNF-related matrix-associated actin-dependent regulator of chromatinsubfamily A member 4
  • BRM/SWI2-related gene 1
  • EC 3.6.1
  • EC 3.6.4.-
  • FLJ39786
  • global transcription activator homologous sequence
  • homeotic gene regulator
  • hSNF2b
  • Mitotic growth and transcription activator
  • nuclear protein GRB1
  • Protein brahma homolog 1
  • Protein BRG-1
  • RTPS2
  • SMARCA4
  • SNF2
  • SNF2B
  • SNF2-beta
  • SNF2L4
  • SNF2LB
  • SNF2-like 4
  • subfamily a, member 4
  • sucrose nonfermenting-like 4
  • SWI/SNF related, matrix associated, actin dependent regulator of chromatin
  • SWI2
  • transcription activator BRG1

Background

Brahma-related gene 1 (BRG1) is a transcriptional activator belonging to the SNF2/RAD54 helicase family, and acting as a component of the SWI/SNF ATP-dependent chromatin remodeling complexes family. BRG1 contains 4 domains of interest; a bromo domain, a helicase ATP-binding domain, a helicase C-terminal domain, and a HAS domain. It is a component of several different complexes with distinctive roles such as the CREST-BRG1 complex which regulates the activation of promoters in a calcium-dependent manner, releasing repressor complexes and recruiting activator complexes. It is also a part of the nBAF and npBAF complexes which are involved in the chromatin remodeling of mononucleosomes and nucleosomal arrays. As a component of the SWI/SNF complex, BRG1 has a role in tumor suppression and has been shown to regulate cell cycle and differentiation when present in neoplasma.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol SMARCA4
Entrez