BRD2 Antibody

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Western Blot: BRD2 Antibody [NB100-260] - RIPA extract (10 ug) from HeLa cells that was untreated (-) or treated (+) with CIP (calf intestinal phosphatase). Antibody used at 0.05 ug/ml followed by rabbit anti-goat HRP.
Immunocytochemistry/ Immunofluorescence: BRD2 Antibody [NB100-260] - PLA uses paired antibodies, so please refer to the PLA page of our website for specific imaging obtained by pairing this antibody with other qualified ...read more
Proximity Ligation Assay: BRD2 Antibody [NB100-260] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human BRD2 (NB100-260) and rabbit anti-human BAF53A (NB100-61628). Image merged from DAPI (2ms) and Texas ...read more
Proximity Ligation Assay: BRD2 Antibody [NB100-260] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human BRD2 (NB100-260) and rabbit anti-human BRD2 (NBP1-30475). Image merged from DAPI (2ms) and Texas ...read more
Proximity Ligation Assay: BRD2 Antibody [NB100-260] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human BRD2 (NB100-260) and rabbit anti-human E2F1 (NB100-2349). Image merged from DAPI (2ms) and Texas ...read more
Proximity Ligation Assay: BRD2 Antibody [NB100-260] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human BRD2 (NB100-260) and rabbit anti-human E2F4 (NBP1-21374). Image merged from DAPI (2ms) and Texas ...read more
Proximity Ligation Assay: BRD2 Antibody [NB100-260] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human BRD2 (NB100-260) and rabbit anti-human H2AX (NB100-383). Image merged from DAPI (2ms) and Texas ...read more
Proximity Ligation Assay: BRD2 Antibody [NB100-260] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human BRD2 (NB100-260) and rabbit anti-human RBP2 (NB110-40499). Image merged from DAPI (2ms) and Texas ...read more
Proximity Ligation Assay: BRD2 Antibody [NB100-260] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human BRD2 (NB100-260) and rabbit anti-human NFRKB (NB100-68269). Image merged from DAPI (2ms) and Texas ...read more
Proximity Ligation Assay: BRD2 Antibody [NB100-260] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human BRD2 (NB100-260) and rabbit anti-human p53 (NB200-171). Image merged from DAPI (2ms) and Texas Red ...read more
Proximity Ligation Assay: BRD2 Antibody [NB100-260] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human BRD2 (NB100-260) and rabbit anti-human TERF2 (NB100-2577). Image merged from DAPI (2ms) and Texas ...read more
Proximity Ligation Assay: BRD2 Antibody [NB100-260] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human BRD2 (NB100-260) and rabbit anti-human YY1 (NBP1-46218). Image merged from DAPI (2ms) and Texas ...read more
Proximity Ligation Assay: BRD2 Antibody [NB100-260] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human BRD2 (NB100-260) and rabbit anti-human BRD4 (NBP1-18874). Image merged from DAPI (2ms) and Texas ...read more

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NB100-260
    • Availability
      Product Discontinued

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BRD2 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residues 550 and 600 of human Bromodomain Containing 2 using the numbering given in entry NP_005095.1 (GeneID 6046).
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
BRD2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:10000-1:40000
  • Immunocytochemistry/Immunofluorescence
  • Proximity Ligation Assay 1:1000-1:20000
Application Notes
By Western blot, this antibody recognizes 2 bands around 110 and 115 kDa, representing BRD2. This antibody does not work for immunoprecipitation.
Positive Control
HeLa Lysate (NB800-PC1)

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for BRD2 Antibody

  • bromodomain containing 2
  • bromodomain-containing 2
  • bromodomain-containing protein 2
  • D6S113E
  • female sterile homeotic-related gene 1
  • FSRG1FSH
  • KIAA9001FLJ31942
  • NAT
  • O27.1.1
  • Really interesting new gene 3 protein
  • RING3DKFZp686N0336
  • RNF3

Background

BRD2 (bromodomain-containing 2) is a nuclear mitogen-activated kinase that plays a role in transcription of cell-cycle-regulated genes by functioning as an adapter protein that recruits E2F and chromatin remodeling factors to E2F response elements. Alternate names of BRD2 include RING3, NAT, RNF3, FSRG1, D6S113E, FLJ31942, DKFZp686N0336, and KIAA9001.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional BRD2 Products

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Bioinformatics

Gene Symbol BRD2
Entrez
Uniprot