BPTF/FALZ RNAi

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications RNAi, RNAi SP

Order Details

BPTF/FALZ RNAi Summary

Specificity
fetal Alzheimer antigen (FALZ), transcript variant 2, mRNA
Gene
BPTF

Applications/Dilutions

Application Notes
This RNAi causes protein knockdown.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for BPTF/FALZ RNAi

  • bromodomain and PHD domain transcription factor
  • Bromodomain and PHD finger-containing transcription factor
  • bromodomain PHD finger transcription factor
  • EC 3.6.1
  • EC 6.2.1.5
  • FAC1fetal Alz-50 reactive clone 1
  • FALZnucleosome-remodeling factor subunit BPTF
  • Fetal Alz-50 clone 1 protein
  • Fetal Alzheimer antigennucleosome remodeling factor, large subunit
  • NURF301

Background

Chimera RNA interference (chimera RNAi) is process by which small interfering RNA/DNA chimera triggers the destruction of mRNA for the original gene.  The discovery work, design, and application of chimera RNAi has been pioneered by Professor Kaoru Saigo and Dr. Kumiko Ui-Tei at the University of Tokyo.  Chimera RNAi has many advantages over the conventional siRNAs.  First, it has been demonstrated to have reliable knock-down for over 10,000 human genes.  Because the human genome is composed of an intricate, genetic network, chimera RNAi's unique design has successfully obviated the off-target effects including microRNA-based influence.  Another advantage of the chimera RNAi technology is its effectiveness at low concentrations (0.5nM to 5nM); only mRNA is destroyed so genomic genes are not affected.  Finally, having both the sense and anti-sense strands consisting RNA/DNA chimera, it offers much greater compound stability for streamlining in vitro and in vivo assays and applications while minimizing interferon induction and other adverse reactions.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. RNAi are guaranteed for 3 months from date of receipt.

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FAQs for BPTF/FALZ RNAi (H00002186-R01). (Showing 1 - 1 of 1 FAQ).

  1. I am interesting by anti-BPTF antibody. I would like to know if a large protein like that could be revealed in PVDF membrane?
    • Regarding your question, nucleosome-remodeling factor subunit (BPTF) is a very large protein as you have also mentioned (338KD). There are some tips for efficient transfer of these proteins in western blot for instance: 1) Make sure to use a low percentage gel, for BPTF in particular I suggest you use 7% gel or even lower (5%). 2) You can use either Nitrocellulose or PVDF, to my knowledge the binding efficiency is higher for nitrocellulose while PVDF is a better choice of membrane if you are planning to do re-probing and stripping. If you are going to use PVDF, make sure to wet it in 100% methanol before use. Furthermore, PVDF membrane exhibits better binding efficiency of electroblotted material in the presence of SDS in the transfer buffer. However SDS added to facilitate transfer of large proteins should not exceed 0.05% as it will interfere with the binding of the protein to the membrane. 3) For transfer you can either use the semi-dry or wet transfer but you decide doing wet transfer, I strongly recommend you doing the transfer in low voltage (at 50v) for instance in order to obtain an efficient transfer. These are some suggestions I can provide for a better and more efficient transfer for large proteins like BPTF.

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Bioinformatics

Gene Symbol BPTF