Boll Antibody (1G8)

Images

 
Western Blot: Boll Antibody (1G8) [H00066037-M04] - Analysis of BOLL expression in transfected 293T cell line by BOLL monoclonal antibody (M04), clone 1G8. Lane 1: BOLL transfected lysatE (31.301 KDa). Lane 2: ...read more
ELISA: Boll Antibody (1G8) [H00066037-M04] - Detection limit for recombinant GST tagged BOLL is approximately 0.3ng/ml as a capture antibody.

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      H00066037-M04
    • Availability
      Product Discontinued

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Boll Antibody (1G8) Summary

Immunogen
BOLL (NP_149019, 185 a.a. ~ 284 a.a) partial recombinant protein with GST tag.ATTQYLPGQWQWSVPQPSASSAPFLYLQPSEVIYQPVEIAQDGGCVPPPLSLMETSVPEPYSDHGVQATYHQVYAPSAITMPAPVMQPEPIKTVWSIHY
Specificity
BOLL (1G8)
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
BOLL
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500
  • ELISA
Application Notes
Antibody reactive against transfected lysate and recombinant protein for western blot. It has also been used for ELISA.

Reactivity Notes

Human. Other species not tested.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Boll Antibody (1G8)

  • bol, boule-like (Drosophila)
  • BOULEbol (Drosophila boule homolog)-like
  • protein boule-like

Background

This gene belongs to the DAZ gene family required for germ cell development. It encodes an RNA-binding protein which is more similar to Drosophila Boule than to human proteins encoded by genes DAZ (deleted in azoospermia) or DAZL (deleted in azoospermia-like). Loss of this gene function results in the absence of sperm in semen (azoospermia). Histological studies demonstrated that the primary defect is at the meiotic G2/M transition. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol BOLL
Entrez
OMIM
Uniprot