BNIP3 Antibody

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Western Blot: BNIP3 Antibody [NBP1-31255] - Sample (30 ug of whole cell lysate) A: 293T 12% SDS PAGE diluted at 1:500

Product Details

Summary
Reactivity Hu, Mu, Rt, BvSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.96 mg/ml

Order Details

BNIP3 Antibody Summary

Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human BNIP3. The exact sequence is proprietary.
Localization
Mitochondrion membrane, Single-pass membrane protein, Mitochondrion membrane
Predicted Species
Mouse (100%), Rat (100%), Bovine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
BNIP3
Purity
Immunogen affinity purified
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Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
Theoretical MW
22 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 10% Glycerol
Preservative
0.01% Thimerosal
Concentration
0.96 mg/ml
Purity
Immunogen affinity purified

Alternate Names for BNIP3 Antibody

  • BCL2/adenovirus E1B 19 kDa protein-interacting protein 3
  • BCL2/adenovirus E1B 19kDa interacting protein 3
  • BCL2/adenovirus E1B 19kD-interacting protein 3
  • BNIP3
  • Nip3

Background

BNIP3, formerly NIP3 (nineteen kDa interacting protein-3), is a pro-apoptotic, mitochondrial protein classified in the Bcl 2 family based on limited sequence homology to the Bcl 2 homology 3 (BH3) domain (amino acids 110-118) and C-terminal TM domain. BNIP3 expressed in yeast and mammalian cells interacts with survival promoting proteins Bcl 2, Bcl XL, CED9 and the adenovirus E1B 19K protein. Typically the BH3 domain of pro-apoptotic Bcl-2 homologues mediates Bcl 2/Bcl XL heterodimerization and confers pro-apoptotic activity. BNIP3 represents a subfamily of Bcl 2 related proteins, which functions without a typical BH3 domain to regulate apoptosis from both mitochondrial and nonmitochondrial sites by selective Bcl 2/Bcl XL interactions. The N-terminus (residues 1-49) and the C-terminus TM domain of BNIP3 are critical for Bcl 2 heterodimerization, and either region is sufficient for Bcl XL interaction. The TM domain of BNIP3 is critical for homodimerization, pro-apoptotic function, and mitochondrial targeting. BNIP3 contains PEST sequences suggesting that the protein may be susceptible to rapid degradation by proteases. PEST sequences commonly contain high local concentrations of amino acids P, E, S, T, and D flanked by charged amino acids and these are abundantly present in NIP3. Thus, the posttranslational control of BNIP3 expression through rapid protein degradation may constitute a mechanism for regulating the intracellular levels of a potentially lethal protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for BNIP3 Antibody (NBP1-31255)

Discover related pathways, diseases and genes to BNIP3 Antibody (NBP1-31255). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Blogs on BNIP3.

BNIP3 - a regulator of mitochondrial autophagy and cell death
Bcl-2 nineteen-kilodalton interacting protein 3 (BNIP3) is a pro-apoptotic BH3-only protein. BNIP3 localizes to the mitochondrial membrane where it plays a key role in mitochondrial autophagy and cell death pathways. Similar to other Bcl-2 family m...  Read full blog post.

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Bioinformatics

Gene Symbol BNIP3
Entrez