Blood Group Lewis b Overexpression Lysate (Native)

Images

 
Western Blot: Blood Group Lewis b Overexpression Lysate (Adult Normal) [NBL1-10865] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Blood Group Lewis b.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

Blood Group Lewis b Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC211069 and based on accession number NM_000149. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens fucosyltransferase 3 (galactoside 3(4)-L-fucosyltransferase, Lewis blood group) (FUT3), transcript variant 1, mRNA.
Gene
FUT3

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
41.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Blood Group Lewis b

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for Blood Group Lewis b Overexpression Lysate (Native)

  • alpha-(1,3/1,4)-fucosyltransferase
  • Blood group Lewis alpha-4-fucosyltransferase
  • CD174
  • EC 2.4.1
  • EC 2.4.1.65
  • FT3B
  • fucosyltransferase 3 (galactoside 3(4)-L-fucosyltransferase, Lewis blood group)
  • Fucosyltransferase 3
  • Fucosyltransferase III
  • FucT-III
  • galactoside 3(4)-L-fucosyltransferase
  • LEfucosyltransferase 3 (galactoside 3(4)-L-fucosyltransferase, Lewis blood groupincluded)
  • Les
  • Lewis FT
  • MGC131739

Background

The Lewis histo-blood group system comprises a set of fucosylated glycosphingolipids that are synthesized by exocrine epithelial cells and circulate in body fluids. The glycosphingolipids function in embryogenesis, tissue differentiation, tumor metastasis, inflammation, and bacterial adhesion. They are secondarily absorbed to red blood cells giving rise to their Lewis phenotype. This gene is a member of the fucosyltransferase family, which catalyzes the addition of fucose to precursor polysaccharides in the last step of Lewis antigen biosynthesis. It encodes an enzyme with alpha(1,3)-fucosyltransferase and alpha(1,4)-fucosyltransferase activities. Mutations in this gene are responsible for the majority of Lewis antigen-negative phenotypes. Multiple alternatively spliced variants, encoding the same protein, have been found for this gene. [provided by RefSeq]. Transcript Variant: This variant (1), also known as minor I, represents the longest transcript. Variants 1, 2, 3, and 4 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for Blood Group Lewis b Lysate (NBL1-10865) (0)

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Bioinformatics

Gene Symbol FUT3