Blood Group Lewis b Antibody (2-25LE)

Images

 
Immunohistochemistry: Blood Group Lewis b Antibody (2-25LE) [NB500-526] - Immunohistochemistry staining of human colon adenocarcinoma (paraffin sections) using anti-blood group Lewis b (clone 2-25LE).
Immunohistochemistry: Blood Group Lewis b Antibody (2-25LE) [NB500-526] - Staining of human small intestine (paraffin sections) using anti-blood group Lewis b (clone 2-25LE).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, IHC, IHC-P
Clone
2-25LE
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Blood Group Lewis b Antibody (2-25LE) Summary

Immunogen
Mucin isolated by size exclusion chromatography from an ovarian cyst fluid (pure endocervical type according to the Fenoglio's classification).
Specificity
The antibody 2-25LE reacts with Lewisb blood group antigen, a carbohydrate determinant carried on both glycolipids and glycoproteins, detected on erythrocytes, certain epithelial cells, and in secretions of certain individuals.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
FUT3
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • ELISA 1:2000
  • Immunohistochemistry 5-10 ug/ml
  • Immunohistochemistry-Paraffin 5 - 10 ug/ml
Application Notes
Purity is > 95% (by SDS-PAGE). Heat mediated antigen retrieval (sodium citrate); mAb incubation 1 hour / RT; detection DAB
Publications
Read Publications using NB500-526.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS (approx. pH 7.4)
Preservative
15mM Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein A purified

Alternate Names for Blood Group Lewis b Antibody (2-25LE)

  • alpha-(1,3/1,4)-fucosyltransferase
  • Blood group Lewis alpha-4-fucosyltransferase
  • CD174
  • EC 2.4.1
  • EC 2.4.1.65
  • FT3B
  • fucosyltransferase 3 (galactoside 3(4)-L-fucosyltransferase, Lewis blood group)
  • Fucosyltransferase 3
  • Fucosyltransferase III
  • FucT-III
  • galactoside 3(4)-L-fucosyltransferase
  • LEfucosyltransferase 3 (galactoside 3(4)-L-fucosyltransferase, Lewis blood groupincluded)
  • Les
  • Lewis FT
  • MGC131739

Background

The antibody 2-25LE reacts with Lewisb blood group antigen, a carbohydrate determinant carried on both glycolipids and glycoproteins, detected on erythrocytes, certain epithelial cells, and in secretions of certain individuals.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Blood Group Lewis b Antibody (NB500-526)(2)

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Bioinformatics

Gene Symbol FUT3
Entrez
Uniprot