Bim Antibody

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Immunohistochemistry-Paraffin: Bim Antibody [NB120-15184] - Human placenta

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NB120-15184
    • Availability
      Product Discontinued

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Bim Antibody Summary

Immunogen
Synthetic peptide (Human) (N terminal).
Localization
Intracytoplasmic membranes
Specificity
Bim
Clonality
Polyclonal
Host
Rabbit
Gene
BCL2L11
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1-2 ug/ml
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:25
Application Notes
Perform enzymatic antigen retrieval before commencing with the IHC staining protocol, by boiling the tissue sections in 10mM citrate buffer,pH 6.0 for 10 minutes, followed by cooling at RT for 20 minutes. Predicted molecular weight: 17 kDa.
Positive Control
HeLa Lysate (NB800-PC1)
Placenta Lysate (NB820-59248)

Reactivity Notes

Cross-reacts with Human, Mouse and Rat. Not yet tested in other species.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
7.0 ml prediluted antibody with 10mM PBS (pH 7.4) and BSA
Preservative
0.1% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Bim Antibody

  • BAM
  • bcl-2 interacting mediator of cell death
  • bcl-2 interacting protein Bim
  • Bcl2-interacting mediator of cell death
  • bcl2-L-11
  • BCL2-like 11 (apoptosis facilitator)
  • bcl-2-like protein 11
  • bcl-2-related ovarian death agonist
  • BIM-alpha6
  • BIM-beta6
  • BIM-beta7
  • BIMBOD
  • BimEL
  • BimL

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Bim Antibody (NB120-15184) (0)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Bim Antibody (NB120-15184). (Showing 1 - of FAQ).

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional Bim Products

Bioinformatics Tool for Bim Antibody (NB120-15184)

Discover related pathways, diseases and genes to Bim Antibody (NB120-15184). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for Bim Antibody (NB120-15184)

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Pathways for Bim Antibody (NB120-15184)

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PTMs for Bim Antibody (NB120-15184)

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Research Areas for Bim Antibody (NB120-15184)

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Blogs on Bim.

Apoptosis and Necroptosis Part I: Important factors to identify both types of programmed cell death
Different types of cell death have classically been identified by discrete morphological changes. The hallmarks of apoptosis include cell shrinkage, nuclear fragmentation and membrane blebbing whereas necroptosis is characterized by cell swelling...  Read full blog post.

Pathway Highlight: Which caspase substrates contribute to the morphological features associated with apoptosis?
Apoptosis, or programmed cell death, is controlled by a caspase signal cascade that activates downstream signals to induce the morphological changes used to differentiate apoptosis from other forms of cell death.  Novus Biologicals offers a variet...  Read full blog post.

Altered expression of BCL2 in cancer
Similar to other cell processes, the balance between cell survival and cell death is an important equilibrium that when altered expression of genes can lead to a variety of disease.  For example, too little cell death can promote cell overgrowth a...  Read full blog post.

IRE1 alpha dependent apoptotic-signaling pathway
Despite in depth characterization of the role of IRE1 alpha (inositol-requiring enzyme 1 alpha) in activating the unfolded protein response (UPR) in the ER - little is known about the molecular mechanisms by which this ER protein has shown to reg...  Read full blog post.

Bcl-2 - an antiapoptotic protein with an important role in cancer cell survival
B-cell lymphoma 2 (Bcl-2) protein is an oncogene that normally acts as an apoptotic inhibitor and localizes to the mitochondrial membrane where it prevents the release of cytochrome c. The Bcl-2 protein family consists of over 20 proteins each co...  Read full blog post.

Understanding Noxa Regulation of Apoptosis
Noxa is a pro-apoptotic gene belonging to the Bcl2 protein family that is unique in that it contains only BH3 domain. The BH3-only subclass of proteins, including proteins like PUMA and Bim in addition to Noxa, regulate the remaining Bcl-2 family memb...  Read full blog post.

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Bioinformatics

Gene Symbol BCL2L11
Entrez