BID Antibody


Western Blot: BID Antibody [NBP1-77150] - Analysis of Bid in mouse lung cell lysates with anti-Bid (CT) using IMG-5613 at (A) 0.5, (B) 1, and (C) 2 ug/ml.
Immunohistochemistry-Paraffin: BID Antibody [NBP1-77150] - Immunohistochemical staining of mouse lung tissue using anti-Bid (CT) at 2 ug/ml.
Immunofluorescence: BID Antibody [NBP1-77150] - Mouse Lung cells at 10 ug/mL.

Product Details

Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA, IHC, IHC-P, IF
1 mg/ml

Order Details

BID Antibody Summary

Antibody was raised against a peptide corresponding to 14 amino acids near the carboxy-terminus of human Bid.
Ion exchange chromatography
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Test in a species/application not listed above to receive a full credit towards a future purchase.

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.02% Sodium Azide
1 mg/ml
Ion exchange chromatography


  • Western Blot 1:100-1:2000
  • ELISA 1:100-1:2000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunofluorescence 10 ug/ml
Application Notes
Bid antibody can be used for detection of Bid by Western blot at 0.5 to 1 mg/ml.
Control Peptide
BID Peptide (NBP1-77150PEP)

Reactivity Notes

Human, Mouse

Alternate Names for BID Antibody

  • apoptic death agonist
  • BH3 interacting domain death agonist
  • BH3-interacting domain death agonist
  • BID isoform ES(1b)
  • BID isoform L(2)
  • BID isoform Si6
  • desmocollin type 4
  • FP497
  • Human BID coding sequence
  • MGC15319
  • MGC42355
  • p22 BID


Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells. Disruption of this process has been implicated in a variety of diseases such as cancer. The Bcl-2 family of proteins is comprised of critical regulators of apoptosis that can be divided into two classes: those that inhibit apoptosis and those that promote cell death. Bid, a pro-apoptotic Bcl-2 family member, is cleaved by caspase-8 in response to apoptotic signals, exposing the Bcl-2 homology 3 (BH3) domain which is normally buried in the full-length protein. The cleaved complex is myris-toylated and translocated to the mitochondrial membrane where it may induce mitochondrial Bax and Bak to oligomerize.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

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Secondary Antibodies


Isotype Controls

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Blogs on BID.

active/cleaved Caspase 2 - Inducing apoptosis in response to cellular stress
Caspase-2 is a highly conserved member of the caspase family involved in the initiation and execution of apoptosis. While its function is still poorly understood, caspase-2 is thought to be important for apoptosis in response to DNA damage, bacteri...  Read full blog post.

Bcl-2 - an antiapoptotic protein with an important role in cancer cell survival
B-cell lymphoma 2 (Bcl-2) protein is an oncogene that normally acts as an apoptotic inhibitor and localizes to the mitochondrial membrane where it prevents the release of cytochrome c. The Bcl-2 protein family consists of over 20 proteins each co...  Read full blog post.

Contact Information

Product PDFs


Gene Symbol BID

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