Recombinant Human Betacellulin/BTC Protein

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE
Concentration
Lyoph

Order Details

Recombinant Human Betacellulin/BTC Protein Summary

Description
Recombinant bioactive protein containing 80 amino acids for Human Betacellulin/BTC

Source:E. coli
Amino Acid Sequence:(P35070)

Preparation
Method
Determined to be >97% pure by SDS-PAGE
Details of Functionality
The ED50, calculated by the dose-dependant proliferation of murine BALB\C 3T3 cells (measured by 3H-thymidine uptake) is < 0.05 ng/ml; corresponding to a Specific Activity of > 2.0 x107IU/mg.
Source
E. coli
Protein/Peptide Type
Biologically Active Protein
Gene
BTC
Purity
>95%, by SDS-PAGE

Applications/Dilutions

Theoretical MW
9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Buffer
Lyophilized after extensive dialysis against 20 mM phosphate buffer, pH 7.4.
Concentration
Lyoph
Purity
>95%, by SDS-PAGE
Reconstitution Instructions
Centrifuge the vial prior to opening. Reconstitute in sterile distilled H2O to a concentration> 100 ug/ml. This solution can then be diluted into other aqueous buffers.

Alternate Names for Recombinant Human Betacellulin/BTC Protein

  • Betacellulin
  • BTC
  • probetacellulin

Background

Human Betacellulin (BTC) is a potent mitogen for retinal pigment epithelial cells and vascular smooth muscle cells. The effects of betacellulin are probably mediated by the egf receptor and other related receptors. Human Recombinant BTC produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 80 amino acids and having a molecular mass of 9 kDa. BTC was purified by proprietary chromatographic techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 2 years from date of receipt.

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Bioinformatics

Gene Symbol BTC
Entrez