beta Tubulin Antibody (3B3)

Images

 
Western Blot: beta Tubulin Antibody (3B3) [H00203068-M04] - Analysis of TUBB expression in Y-79 (Cat # L042V1).
Immunohistochemistry-Paraffin: beta Tubulin Antibody (3B3) [H00203068-M04] - Analysis of monoclonal antibody to TUBB on formalin-fixed paraffin-embedded human colon. Antibody concentration 3 ug/ml
Sandwich ELISA: beta Tubulin Antibody (3B3) [H00203068-M04] - Detection limit for recombinant GST tagged TUBB is 1 ng/ml as a capture antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, IHC, IHC-P
Clone
3B3
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

beta Tubulin Antibody (3B3) Summary

Immunogen
TUBB (AAH19924, 1 a.a. ~ 445 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.MREIVHIQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLDRISVYYNEATGGKYVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAV
Marker
Microtubule Marker
Specificity
TUBB - tubulin, beta (3B3)
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
TUBB
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500
  • ELISA
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
Application Notes
Antibody reactive against cell lysate and recombinant protein for western blot. It has also been used for ELISA.
Publications
Read Publications using H00203068-M04.

Reactivity Notes

Human.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for beta Tubulin Antibody (3B3)

  • beta polypeptide
  • MGC117247
  • Tubb5
  • tubulin beta chain
  • tubulin beta polypeptide
  • tubulin beta-1 chain
  • Tubulin beta-5 chain
  • tubulin, beta

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for beta Tubulin Antibody (H00203068-M04)(2)

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for beta Tubulin Antibody (H00203068-M04). (Showing 1 - 1 of 1 FAQs).

  1. Our customer want to know which tubulin (alpha or beta) is suitable for WB as the loading control in brain tissue. Could you recommend the tubulin alpha or beta as a loading control for WB in brain tissue? In case of adipose tissue, tubulin alpha is not suitable as a loading control. How about in brain tissue? Could you let me know?
    • Both beta and alpha tubulin are expressed in the brain, and either one would make a suitable loading control. Beta tubulin may give a cleaner signal than alpha, but this varies greatly. You may wish to consider NB600-936.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol TUBB
Entrez
Uniprot