beta-NGF Overexpression Lysate (Native)

Images

 
Western Blot: NGF Overexpression Lysate (Adult Normal) [NBL1-13634] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for NGF.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

beta-NGF Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC221463 and based on accession number NM_002506. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens nerve growth factor (beta polypeptide) (NGF), mRNA.
Gene
NGF

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
24.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for beta-NGF

Type
Overexpression
Tissue
Nervous
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for beta-NGF Overexpression Lysate (Native)

  • Beta nerve growth factor precursor
  • beta-nerve growth factor
  • betaNGF
  • beta-NGF
  • HSAN5
  • MGC161426
  • nerve growth factor (beta polypeptide)
  • nerve growth factor, beta subunit
  • NGFB
  • NGFBMGC161428

Background

This gene is a member of the NGF-beta family and encodes a secreted protein which homodimerizes and is incorporated into a larger complex. This protein has nerve growth stimulating activity and the complex is involved in the regulation of growth and the differentiation of sympathetic and certain sensory neurons. Mutations in this gene have been associated with hereditary sensory and autonomic neuropathy, type 5 (HSAN5), and dysregulation of this gene's expression is associated with allergic rhinitis. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for beta-NGF Lysate (NBL1-13634) (0)

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Bioinformatics

Gene Symbol NGF
Entrez