beta-III Tubulin Antibody (TUJ-1)

Product Discontinued

beta-III Tubulin Antibody (TUJ-1) Summary

• Immunogen: CIII beta-Tubulin protein (Cter domain)
• Marker: Neuronal Marker
• Specificity: The mouse monoclonal reacts specifically with human class III beta-Tubulin (55 kDa)
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: TUBB3
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot 1:200-1:1000

Reactivity Notes

Cross reacts with Human.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Ascites
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Unpurified
• Reconstitution Instructions: Reconstitute with 0.2 ml distilled water.

Alternate Names for beta-III Tubulin Antibody (TUJ-1)

• Beta3-tubulin
• beta-4
• betaIII Tubulin
• beta-III Tubulin
• beta-Tubulin III
• Class III beta-tubulin
• TUBB3
• TUBB4 CFEOM3A
• Tubulin Beta 3
• tubulin beta-3 chain
• Tubulin beta-4 chain
• Tubulin beta-4
• Tubulin beta-III
• tubulin, beta 3
• TUJ1 antigen
• TUJ-1 antigen

Background

Tubulin is the major building block of microtubules. This intracellular cylindrical filamentous structure is present in almost all eukaryotic cells. Microtubules function as structural and mobile elements in mitosis, intracellular transport, flagellar movement, and the cytoskeleton. Except in the simplest eukaryotes, tubulin exists in all cells as a mixture of similar, but not identical, sets of alpha and beta tubulin polypeptides. Within either set of polypeptides, individual subunits diverge from each other (both within and across species) at less than 10percent of the amino acid positions. The most extreme diversity is localized to the 15 residues of the carboxy terminal. For beta tubulin five evolutionarily conserved isotype clones have been identified. These are almost totally conserved in the subunits utilized in the same cell types of different species, with the exception of the hematopoietic beta tubulin which is the most highly divergent in sequence and is not conserved between species. Research has been centered around the hypothesis that these beta tubulin isotypes contribute to unique functional properties. It has been reported that the different isotypes of tubulin differ from each other in their ability to polymerize into microtubules. The monoclonal antibody from hybridoma SDL.3D10 can stimulate microtubule assembly when reconstituted with tubulin, tau or MAP2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for beta-III Tubulin Antibody (NB100-57388)(4)

Publications using NB100-57388

• Elsas J, Sellhaus B, Herrmann M et al. Fetuin-A in the developing brain. Dev Neurobiol 2013-05-01 [PMID: 23109215] (ICC/IF, IHC-P, Rat)
• Yang D, Han Y, Zhang J et al. Therapeutic effect of human umbilical tissue-derived cell treatment in Rats with experimental intracerebral hemorrhage Brain Res 2012-03-20 [PMID: 22341873] (IF/IHC, Rat)
• Seve P, Lai R, Ding K, Winton T et al. Class III beta-tubulin expression and benefit from adjuvant cisplatin/vinorelbine chemotherapy in operable non-small cell lung cancer: analysis of NCIC JBR.10. Clin Cancer Res. 13(3):994-9. 2007-02-01 [PMID: 17289895] (IHC-P, Human)
• Seve P, Reiman T, Lai R et al. Class III beta-tubulin is a marker of paclitaxel resistance in carcinomas of unknown primary site. Cancer Chemother Pharmacol;60(1):27-34. 2007-06-01 [PMID: 17021819]

Bioinformatics

• Gene Symbol: TUBB3
• Entrez:
  • 10381(Human)
• Uniprot:
  • Q13509(Human)