beta-III Tubulin Antibody (5H2)

Images

 
Western Blot: beta-III Tubulin Antibody (5H2) [NBP1-48038] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Tubulin Beta 3 (Right lane) cDNA for 48 hrs and lysed. Equivalent ...read more
Western Blot: beta-III Tubulin Antibody (5H2) [NBP1-48038] - Analysis of extracts (35ug) from 9 different cell lines by using anti-Tubulin Beta 3 monoclonal antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clone
5H2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

beta-III Tubulin Antibody (5H2) Summary

Immunogen
Recombinant protein expressed in E.coli corresponding to amino acids 1-150 of human TUBB3
Marker
Neuronal Marker
Specificity
This antibody is specific for Homo sapiens tubulin, beta 3 (TUBB3).
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
TUBB3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-2000

Reactivity Notes

Human

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for beta-III Tubulin Antibody (5H2)

  • beta-4
  • betaIII Tubulin
  • beta-III Tubulin
  • beta-Tubulin III
  • TUBB4 CFEOM3A
  • Tubulin Beta 3
  • tubulin beta-3 chain
  • Tubulin beta-4 chain
  • Tubulin beta-4
  • Tubulin beta-III
  • tubulin, beta 3
  • TUJ1 antigen
  • TUJ-1 antigen

Background

Tubulin is the major building block of microtubules. This intracellular cylindrical filamentous structure is present in almost all eukaryotic cells. Microtubules function as structural and mobile elements in mitosis, intracellular transport, flagellar movement, and the cytoskeleton. Except in the simplest eukaryotes, tubulin exists in all cells as a mixture of similar, but not identical, sets of alpha and beta tubulin polypeptides. Within either set of polypeptides, individual subunits diverge from each other (both within and across species) at less than 10% of the amino acid positions. The most extreme diversity is localized to the 15 residues of the carboxy terminal. For beta tubulin five evolutionarily conserved isotype clones have been identified. These are almost totally conserved in the subunits utilized in the same cell types of different species, with the exception of the hematopoietic beta tubulin which is the most highly divergent in sequence and is not conserved between species. Research has been centered around the hypothesis that these beta tubulin isotypes contribute to unique functional properties. It has been reported that the different isotypes of tubulin differ from each other in their ability to polymerize into microtubules.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Secondary Antibodies

 

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Bioinformatics Tool for beta-III Tubulin Antibody (NBP1-48038)

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Blogs on beta-III Tubulin.

Beta Tubulin III and neurogenesis
Beta tubulin III, also known as Tuj-1, is a class III member of the beta tubulin protein family. Beta tubulins are one of two structural components that form our microtubule network. While general tubulins play a role in a wide range of cellular pr...  Read full blog post.

Beta III tubulin
The Beta III tubulin protein is abundantly present in both the central and peripheral nervous systems (CNS and PNS), where it is predominantly expressed during fetal and postnatal development. In cerebellar and sympathoadrenal neurogenesis, Beta III d...  Read full blog post.

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Bioinformatics

Gene Symbol TUBB3
Entrez