beta II Tubulin B Antibody (5B3)

Images

 
Western Blot: beta II Tubulin B Antibody (AT5B3) [NBP1-74029] - PAGE, transferred to PVDF membrane and probed with anti-human TUBB2b (1:500). Proteins were visualized using a goat anti-mouse secondary antibody ...read more
Immunocytochemistry/ Immunofluorescence: beta II Tubulin B Antibody (AT5B3) [NBP1-74029] - Immunofluorescence of human SW480 cells stained with monoclonal anti-human TUBB2b antibody (1:250) with Texas Red (Red). Arrow ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA, ICC/IF
Clone
5B3
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

beta II Tubulin B Antibody (5B3) Summary

Immunogen
Recombinant human TUBB2b (1-445aa) purified from E. coli
Specificity
Anti-human TUBB2b mAb, clone 5B3, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human TUBB2b protein.
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
TUBB2B
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:250
Application Notes
The antibody has been tested by ELISA, Western blot, and Immunofluorescence analyses to assure specificity and reactivity. Since applications vary, however, each investigation should be titrated by a reagent to obtain optimal results. Recommended dilution range for Western blot analysis and Immunofluorescence is 1:250 ~ 500. Recommended starting dilution is 1:250

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4) and 10% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for beta II Tubulin B Antibody (5B3)

  • bA506K6.1
  • class II beta-tubulin isotype
  • DKFZp566F223
  • FLJ98847
  • MGC8685
  • tubulin beta-2B chain
  • tubulin, beta 2B
  • tubulin, beta polypeptide paralog

Background

The protein encoded by this gene is a beta isoform of tubulin, which binds GTP and is a major component of microtubules. Multiple beta Tubulin isoforms have been characterized and are expressed in mammalian tissues. Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. TUBB2b is mainly expressed in developing neurons with a dominant expression during the critical steps of corticogenesis. Also, TUBB2B is a new critical gene involved in neuronal migration. It is present in the nuclei and nucleoplasm, Defects in this gene are a cause of asymmetric polymicrogyria.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP1-74029AF405
Alexa Fluor 488 Labeled NBP1-74029AF488
Alexa Fluor 647 Labeled NBP1-74029AF647
Alexa Fluor 700 Labeled NBP1-74029AF700
DyLight 350 Labeled NBP1-74029UV
DyLight 405 Labeled NBP1-74029V
DyLight 488 Labeled NBP1-74029G
DyLight 680 Labeled NBP1-74029FR
DyLight 755 Labeled NBP1-74029IR

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Bioinformatics

Gene Symbol TUBB2B
Uniprot