beta II Tubulin A Antibody (2-28-33)

Images

 
Western Blot: beta II Tubulin A Antibody (2-28-33) [NB120-11311] - Whole cell extract of Chicken fibroblasts was separated on SDS-PAGE, and blotted with monoclonal Anti-beta Tubulin. The antibody was developed with Goat ...read more
Immunocytochemistry/ Immunofluorescence: beta II Tubulin A Antibody (2-28-33) [NB120-11311] - Double immunofluorescent labeling PTK-1 cells in anaphase and interphase stained with Monoclonal and Anti-beta-Tubulin (Goat ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Ch, MaSpecies Glossary
Applications WB, ICC/IF
Clone
2-28-33
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

beta II Tubulin A Antibody (2-28-33) Summary

Immunogen
Sarkosyl-resistant ribbons from Strongylocentrotus purpuratus (sea urchin) sperm axonemes.
Localization
Major constituent of microtubules.
Specificity
The antibody recognizes the two major and one of the minor b tubulin isotypes of the free-living soil nematode C. elegans. It also binds to Sarkosyl-resistant ribbons from Strongylocentrotus purpuratus sperm axonemes, to neuronal axons of the larva of an ascidian (Ciona intestinalis) and to tubulin of chicken and mammals, cultured human fibroblasts, bovine and rat brain tissue. In C.elegans preparations, the antibody does not recognize tubulin that has been heat denatured in the presence of SDS and beta-mercaptoethanol, though it is bound to tubulin separated on isoelectric focusing gels. It reacts with heat-denatured and reduced mammalian preparations in immunoblotting.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
TUBB2A
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:10-1:2000
Application Notes
ICC: Use at an assay dependent dilution. IF: Use at a dilution of 1/1000, this dilution was determined by indirect immunofluorescent labeling of cultured human fibroblasts. Western Blot: Use at an assay dependent dilution. Predicted molecular weight: 50 kDa. Optimal dilutions/concentrations should be determined by the end user.

Reactivity Notes

Cross-reacts with all Mammals, Chicken and Sea Urchin. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Raw ascites
Preservative
15mM Sodium Azide
Purity
Unpurified

Alternate Names for beta II Tubulin A Antibody (2-28-33)

  • dJ40E16.7
  • TUBB
  • TUBB2
  • TUBB2B
  • tubulin beta-2A chain
  • tubulin, beta 2
  • tubulin, beta 2A
  • tubulin, beta polypeptide 2
  • tubulin, beta polypeptide

Background

Tubulin is the major building block of microtubules. This intracellular cylindrical filamentous structure is present in almost all eukaryotic cells. Microtubules function as structural and mobile elements in mitosis, intracellular transport, flagellar movement, and in the cytoskeleton.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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ICC/IF Video Protocol

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Bioinformatics

Gene Symbol TUBB2A