beta I Tubulin Antibody (SAP.4G5)

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Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, Ca, Ch, XpSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P
Clone
SAP.4G5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

beta I Tubulin Antibody (SAP.4G5) Summary

Immunogen
Synthetic peptide corresponding to the C-terminal sequence of beta-tubulin isotype I, coupled to BSA.
Epitope
NB 120-11312 specifically recognizes an epitope located in the C-terminal sequence of beta tubulin isotype I of human, bovine, dog, hamster, rat, mouse, chicken and Xenopus.
Localization
Major constituent of microtubules.
Specificity
No reactivity with other tubulin isotypes is observed.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
TUBB1
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:20000
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
Application Notes
ICC: Use (methanolacetone and 3% paraformaldehyde-0.5% Triton-x-100 fixations). IHC: Use 10% neutral buffered formalin-alcoholic formalin-xylene, paraffin-embedded. WB: Predicted molecular weight: 55kDa.
Positive Control
beta I Tubulin Lysate (NBL1-17436)

Reactivity Notes

Cross-reacts with Human, Mouse, Rat, Xenopus laevis, Cow, Chicken and Dog. Not yet tested in other species. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Raw ascites
Preservative
15mM Sodium Azide
Purity
Unpurified

Alternate Names for beta I Tubulin Antibody (SAP.4G5)

  • beta tubulin 1, class VI
  • betaI Tubulin
  • beta-I Tubulin
  • dJ543J19.4
  • TUBB1
  • tubulin beta-1 chain
  • tubulin, beta 1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for beta I Tubulin Antibody (NB120-11312) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for beta I Tubulin Antibody (NB120-11312)

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Bioinformatics

Gene Symbol TUBB1
Entrez