beta Galactosidase Antibody [HRP]


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Product Details

Reactivity Mu, BaSpecies Glossary
Applications WB, ELISA, IHC, IHC-P
Please see the vial label for concentration. If unlisted please contact technical services.

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Available Conjugates
Catalog# & Conjugate Size Price

beta Galactosidase Antibody [HRP] Summary

This antibody is purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
Recombinant full length protein (E. coli).
Ion exchange chromatography
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Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
20mM Potassium Phosphate, 150 mM NaCl, pH 7.2, with10 mg/mL BSA (BSA) - Immunoglobulin and Protease free
0.01% Gentamicin Sulfate
Ion exchange chromatography


  • Western Blot 1:2000-1:5000
  • ELISA 1:10000-1:50000
  • Immunohistochemistry 1:500-1:2000
  • Immunohistochemistry-Paraffin 1:10-1:500
Application Notes
Suitable for immunoblotting, ELISA, Immunofluorescence microscopy, immunoprecipitation, conjugation and most immunological methods requiring high titer and specificity. The antibody recognizes both frozen tissue sections, paraffin embedded tissue and 4% paraformaldehyde fixed tissue for most immunohistochemical analysis. A 1:5,000 dilution has been reported - be successful for staining by immunoblot of beta-galactosidase fusion proteins after transfer using a semi-dry transfer apparatus. A 1:1,500 dilution has been reported - detect beta-galactosidase in adult rat spinal cord tissue after infection with helper-dependent adenovirus expressing lacZ. In this particular experiment, tissue was perfused with 4% paraformaldehyde and cryostat-cut - produce free-floating sections. A 1:5,000 dilution has been reported - be successful for staining of brain sections from transgenic mice expressing nuclear beta-galactosidase when assayed by Immunofluorescence microscopy. A 1:5,000 dilution has been reported for immunofluorescent staining of methanol fixed, devitellinized Drosophila embryos. Although a wide range of conditions was reported - be effective, a 1:10,000 dilution was noted - show no background and - be suitable for double labeling experiments.
Readout System

Reactivity Notes

Cross-reacts with E. coli.

Alternate Names for beta Galactosidase Antibody [HRP]

  • EC; b-gal


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies


Isotype Controls

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