beta-Catenin Antibody (8E7) [Alexa Fluor® 405]

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Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P
Clone
8E7
Clonality
Monoclonal
Host
Mouse
Conjugate
Alexa Fluor 405

beta-Catenin Antibody (8E7) [Alexa Fluor® 405] Summary

Immunogen
Fusion protein corresponding to residues 1-100 of human beta-catenin with a c-terminal histidine tag
Epitope
The epitope corresponds to amino acid residues 36-44 (HSGATTTAP) of human beta-catenin.
Marker
Epithelial Cell Marker, Adherens Junction Marker
Specificity
Recognizes active form of beta-catenin, dephosphorylated on Ser37 or Thr41.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
CTNNB1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
Application Notes
This antibody is useful in Flow Cytometry, Immunocytochemistry, Immunohistochemistry-Paraffin, Western Blot. In Immunocytochemistry this antibody showed positive staining on the membrane and cytosol.

Reactivity Notes

Human, Mouse, Rat. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Alternate Names for beta-Catenin Antibody (8E7) [Alexa Fluor® 405]

  • bCatenin
  • beta 1 (88kD)
  • beta-Catenin
  • catenin (cadherin-associated protein), beta 1, 88kDa
  • catenin beta-1
  • CTNNB
  • CTNNB1
  • DKFZp686D02253
  • FLJ25606
  • FLJ37923

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, Flow, ICC/IF, IHC, IHC-P

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for beta-Catenin Antibody (NBP1-71720AF405) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 488 Labeled NBP1-71720AF488
Alexa Fluor 647 Labeled NBP1-71720AF647
Alexa Fluor 700 Labeled NBP1-71720AF700

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Blogs on beta-Catenin.

Epithelial-Mesenchymal Transition (EMT) Markers
Epithelial-Mesenchymal Transition (EMT) is the trans-differentiation of stationary epithelial cells into motile mesenchymal cells. During EMT, epithelial cells lose their junctions and apical-basal polarity, reorganize their cytoskeleton, undergo a...  Read full blog post.

CD63: is it pro-metastatic or anti-metastatic?
CD63 is a type II membrane protein belonging to tetraspanin superfamily and it play key roles in the activation of several cellular signaling cascades along with acting as TIMP1 receptor. It is expressed by activated platelets, monocytes,...  Read full blog post.

Notch1 - A multifunctional transmembrane receptor
Notch1 is a member of the Notch family of Type 1 single-pass transmembrane proteins that share an extracellular domain of multiple epidermal growth factor-like (EGF) repeats. Notch family members play key roles in a variety of developmental process...  Read full blog post.

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Bioinformatics

Gene Symbol CTNNB1
Entrez

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