beta-Catenin Antibody (4D5)

Images

 
Western Blot: beta-Catenin Antibody (4D5) [NBP1-51588] - Western blot analysis using CTNNB1 mouse mAb against CTNNB1-hIgGFc transfected HEK293 cell lysate.
Immunocytochemistry/ Immunofluorescence: beta-Catenin Antibody (4D5) [NBP1-51588] - Immunofluorescence analysis of A549 (left) and SK-BR-3 (right) cells using CTNNB1 mouse mAb (green). Red: Actin filaments have been ...read more
Immunohistochemistry-Paraffin: beta-Catenin Antibody (4D5) [NBP1-51588] - Immunohistochemical analysis of paraffin-embedded human Placenta tissues using CTNNB1 mouse mAb
Flow Cytometry: beta-Catenin Antibody (4D5) [NBP1-51588] - Flow cytometric analysis of A549 cells using CTNNB1 mouse mAb (green) and negative control (purple).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC-P
Clone
4D5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

beta-Catenin Antibody (4D5) Summary

Immunogen
Purified recombinant fragment of human CTNNB1 expressed in E. Coli.
Marker
Epithelial Cell Marker, Adherens Junction Marker
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CTNNB1
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:2000
  • ELISA 1:10000
  • Flow Cytometry 1:200-1:400
  • Immunocytochemistry/Immunofluorescence 1:200-1:1000
  • Immunohistochemistry-Paraffin
Theoretical MW
85 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Ascites
Preservative
0.03% Sodium Azide
Purity
Unpurified

Alternate Names for beta-Catenin Antibody (4D5)

  • bCatenin
  • beta 1 (88kD)
  • beta-Catenin
  • catenin (cadherin-associated protein), beta 1, 88kDa
  • catenin beta-1
  • CTNNB
  • CTNNB1
  • DKFZp686D02253
  • FLJ25606
  • FLJ37923

Background

The catenins, a, beta, and ', are proteins that bind to the highly conserved, intracellular cytoplasmic tail of E-cadherin. Together, the catenin/cadherin complexes play critical roles in mediating cellular adhesion. beta-catenin associates with the cytoplasmic portion of E-cadherin, which is necessary for the function of E-cadherin as an adhesion molecule. Beta-catenin also forms complexes with the tumor suppressor protein APC. Amino acid alterations at residues around Ser 33, one of the targets for phosphorylation of glycogen synthase kinase-3beta, result in accumulation of the beta-catenin protein in the cytoplasm and nucleus. Pin1 is a novel regulator of beta-catenin signaling that directly binds a phosphorylated Ser-Pro motif next to the APC-binding site in beta-catenin, inhibiting the interaction with APC and increasing beta-catenin translocation into the nucleus. Thus, Pin1 overexpression may contribute to the upregulation of beta-catenin in tumors such as breast cancer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Discover related pathways, diseases and genes to beta-Catenin Antibody (NBP1-51588). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Blogs on beta-Catenin.

Epithelial-Mesenchymal Transition (EMT) Markers
Epithelial-Mesenchymal Transition (EMT) is the trans-differentiation of stationary epithelial cells into motile mesenchymal cells. During EMT, epithelial cells lose their junctions and apical-basal polarity, reorganize their cytoskeleton, undergo a...  Read full blog post.

CD63: is it pro-metastatic or anti-metastatic?
CD63 is a type II membrane protein belonging to tetraspanin superfamily and it play key roles in the activation of several cellular signaling cascades along with acting as TIMP1 receptor. It is expressed by activated platelets, monocytes,...  Read full blog post.

Notch1 - A multifunctional transmembrane receptor
Notch1 is a member of the Notch family of Type 1 single-pass transmembrane proteins that share an extracellular domain of multiple epidermal growth factor-like (EGF) repeats. Notch family members play key roles in a variety of developmental process...  Read full blog post.

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Bioinformatics

Gene Symbol CTNNB1
Entrez