beta-Actin Antibody (S12-I)

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Western Blot: beta-Actin Antibody (S12-I) [NBP1-33778] - Actin in mouse brain crude lysate (50 ug of protein loaded).

Product Details

Summary
Product Discontinued
View other related beta-Actin Primary Antibodies

Order Details


    • Catalog Number
      NBP1-33778
    • Availability
      Product Discontinued

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beta-Actin Antibody (S12-I) Summary

Immunogen
Peptide derived from C-terminal peptide of human B-actin.
Specificity
This antibody recognizes both alpha and beta actin proteins.
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
ACTB
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2 000
  • ELISA 1:10000-1:20000
  • Immunoprecipitation 1:10-1:500
Application Notes
(For 0.1ml, 0.5ml and 1.0ml sizes, use recommended dilutions. For 7.0ml and 15.0ml sizes, products should be used undiluted). End concentration/dilutions should be determined by end user.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20 mM Tris-HCl, pH 8.0, 10 mg/ml BSA.
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified

Notes

The 7mL and 15 mL sizes that this product is available in is a pre-diluted size and no additional dilutions are required before using this item for the intended application.

Alternate Names for beta-Actin Antibody (S12-I)

  • ACTB
  • actin, beta
  • actin, cytoplasmic 1
  • Beta Actin
  • beta cytoskeletal actin
  • betaActin
  • beta-Actin
  • BRWS1
  • PS1TP5-binding protein 1
  • PS1TP5BP1

Background

Actins are highly conserved proteins that are involved in cell motility, structure and integrity, processes that are crucial for tissue development and the development of organism. The actin cytoskeleton is one of the principal drivers of cell motility and is capable of responding to complex signaling cascades. Recent evidence suggests that it may play key roles in regulating apoptosis and aging. Beta actin is one of six different actin isoforms which have been identified. Like GAPDH, beta-actin is constitutively expressed at high levels in almost all tissues and cell lines making it ideal for use as a loading control marker in immunoblots.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for beta-Actin Antibody (NBP1-33778). (Showing 1 - 4 of 4 FAQ).

  1. Why is beta actin used as a loading control in blotting ?
    • Beta actin is a highly-expressed protein found in all cells, and is considered a house keeping protein whose expression is necessary for any cell's proper functioning. For this reason it is used as a loading control to confirm that the same amount of total protein is loaded in each lane of an SDS-PAGE gel so that appropriate comparisons can be made between the actual proteins of interest in different samples.
  2. Are the beta-Actin antibodies validated in Simple Western?
    • Yes, we offer a few beta actin antibodies that have been tested in Simple Western: NBP1-47423, NB600-501, NB600-532, NB600-503, and NB100-56874.
  3. Do Beta-Actin antibodies come in lyophilized format?
    • ACTB antibodies like MAB8969 AND MAB8929 come in lyophilized form.
  4. What the theoretical molecular weight for Beta-Actin antibodies?
    • The TMW of beta Actin antibodies is approximately 40 - 42 kDa.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for beta-Actin Antibody (NBP1-33778)

Discover related pathways, diseases and genes to beta-Actin Antibody (NBP1-33778). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for beta-Actin Antibody (NBP1-33778)

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PTMs for beta-Actin Antibody (NBP1-33778)

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Research Areas for beta-Actin Antibody (NBP1-33778)

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Blogs on beta-Actin.

The use of Beta Actin (AC-15) as a loading control across multiple species
Actin is a fundamental component of the cytoskeleton, where it has the ability to create and break down actin filament formation in response to various cell needs.  Actin has six highly conserved isoforms, however beta and gamma actin are the two...  Read full blog post.

Tips on choosing an ideal loading control antibody for Western Blotting
Western blotting is one of the most commonly used antibody assay techniques in cell and molecular biology research since its development over three decades ago, and is considered the gold standard for protein detection and quantification. When p...  Read full blog post.

Beta-Actin's Role in Neuronal Plasticity
Beta-Actin is a highly conserved protein involved in cell growth, cytoskeletal and extracellular support structures and cell migration. Because beta-Actin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading contro...  Read full blog post.

ACTB - an abundant cytoskeletal component with applications for gene expression analysis
Actin is the widely studied and ubiquitous cytoskeletal protein capable of forming dynamic microfilament structures. These filaments are essential for diverse cellular functions including cell shape, migration, cytokinesis, and intracellular traffi...  Read full blog post.

A New Standard in Antibody Testing - Simple Western Certified Antibodies
The Western blot is one of the most commonly used antibody assay techniques in cell and molecular biology research since its development over three decades ago, and is considered the gold standard for protein detection and quantification. The tradi...  Read full blog post.

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Bioinformatics

Gene Symbol ACTB
Entrez
Uniprot