beta 2-Microglobulin Antibody (F21-21)

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Flow Cytometry: beta 2-Microglobulin Antibody (F21-21) [NBP1-28311] - Analysis using the FITC conjugate of NBP1-28311. Double staining of Chicken peripheral blood mononuclear cells with mouse anti-chicken beta2- ...read more

Product Details

Summary
Reactivity Av, Ch, TrSpecies Glossary
Applications WB, Flow, IHC, IHC-P, IP, ICC
Clone
F21-21
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

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Catalog# & Conjugate Size Price

beta 2-Microglobulin Antibody (F21-21) Summary

Immunogen
Affinity purified chicken MHC-I molecules
Specificity
The beta2-microglobulin subunit (Mr approx. 14 kDa) of avian MHC Class I molecules
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
B2M
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Flow Cytometry <= 1 ug/10^6 cells
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10-1:500
  • Immunocytochemistry

Reactivity Notes

Chicken and Turkey

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
0.1M BBS (pH 8.2)
Preservative
No Preservative
Concentration
0.5 mg/ml
Purity
Protein A or G purified

Alternate Names for beta 2-Microglobulin Antibody (F21-21)

  • B2M
  • beta 2Microglobulin
  • beta 2-Microglobulin
  • beta chain of MHC class I molecules
  • beta-2-microglobin
  • beta-2-microglobulin

Background

Like their mammalian counterparts, avian MHC Class I molecules (also known as B-F antigens) consist of a highly polymorphic alpha-chain (Mr approx. 44 kDa) non-covalently bound to the invariant beta2-microglobulin subunit (Mr approx. 14 kDa). MHC Class I molecules are expressed on most nucleated cells where they present endogenously synthesized antigenic peptides to CD8+ T lymphocytes, which are usually cytotoxic T cells. (1-2)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Biotin Labeled NBP1-28313
FITC Labeled NBP1-28312

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Bioinformatics

Gene Symbol B2M
Entrez