Human BDNF ELISA Kit (Colorimetric)

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ELISA: Human BDNF ELISA Kit (Colorimetric) [NBP2-59954]

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
serum, plasma, cell culture supernates, tissue homogenates
Standard Curve Range
0.3125 ng/ml-20 ng/ml
Sensitivity
0.063 ng/ml

Order Details

Human BDNF ELISA Kit (Colorimetric) Summary

Specificity
This assay has high sensitivity and excellent specificity for detection of human BDNF. No significant cross-reactivity or interference between human BDNF and analogues was observed.
Standard Curve Range
0.3125 ng/ml-20 ng/ml
Sensitivity
0.063 ng/ml
Assay Type
Sandwich ELISA
Inter-Assay
CV%<10%
Intra-Assay
CV%<8%
Sample Volume
50-100ul
Kit Type
ELISA Kit (Colorimetric)
Gene
BDNF

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8° C. Protect from light. Do not use past expiration date.

Kit Components

Components
  1. Instruction manual
  2. HRP-avidin (100 x concentrate)
  3. HRP-avidin Diluent
  4. Biotin-antibody Diluent
  5. Biotin-antibody (100 x concentrate)
  6. Adhesive Strip (For 96 wells)
  7. Assay plate (12 x 8 coated Microwells)
  8. Sample Diluent
  9. Standard (Freeze dried)
  10. Stop Solution
  11. Wash Buffer (25 x concentrate)
  12. TMB Substrate

Notes

Please contact our Technical Services with any questions regarding species reactivity

Alternate Names for Human BDNF ELISA Kit (Colorimetric)

  • Abrineurin
  • ANON2
  • BDNF
  • brain-derived neurotrophic factor
  • BULN2
  • MGC34632
  • neurotrophin

Background

This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for BDNF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any BDNF present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for BDNF is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of BDNF bound in the initial step. The color development is stopped and the intensity of the color is measured.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol BDNF
Entrez