Bcl3 Antibody

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Western Blot: Bcl3 Antibody [NBP1-45376] - Western blot analysis of BCL-3 in Jurkat cell lysate.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

Bcl3 Antibody Summary

Immunogen
Synthetic peptide surrounding amino acid 424 of human Bcl-.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
BCL3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • Immunohistochemistry
  • Immunoprecipitation
Application Notes
Western blot analysis (2-4 ug/ml). Per researchers feedback, the antibody also works well in Immunoprecipation (10-20 ug/ml), and Immunohistochemistry (20-40 ug/ml). However, the optimal conditions should be determined individually. Detects 55-60 kDa Bcl-3 of human, mouse, and rat origins. Other species have not been tested.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2), 0.5% BSA and 30% Glycerol
Preservative
0.01% Thimerosal
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Bcl3 Antibody

  • B-cell CLL/lymphoma 3
  • B-cell leukemia/lymphoma 3
  • B-cell lymphoma 3 protein
  • B-cell lymphoma 3-encoded protein
  • BCL-3
  • BCL4
  • chronic lymphatic leukemia protein
  • D19S37
  • Proto-oncogene BCL3

Background

The Ham150-3.5 antibody reacts with mouse and human Bcl-3. Bcl-3 is a member of the IkappaB multigene family, which modulates the activities of NF-kappaB/Rel transcription factors. Bcl-3 is reported to increase transcription from NF-kappaB responsible promoters and to dissociate p50-p52 homodimers from DNA. Bcl-3 is phosphorylated influencing its interaction with both p50 and p52. Bcl-3 was cloned from a chromosomal breakpoint in the t(14;19) translocation, which is found in some cases of chronic B-cell lymphocytic leukemias. Bcl-3 is required for T-cell-dependent immunity. Bcl-3-deficient mice are defective in antigen-specific antibody production and germinal-center formation and fail to resist infection. Bcl-3 may also contribute to B-cell survival, which may explain its oncogenic potential when expressed at high levels as result of chromosomal translocation. Bcl-3 is detected in different tissues, especially the spleen and other lymphoid organs. The gene was shown to be induced by mitogenic stimuli in B and T cells and by cytokines on human erythroid precursors. It is shown that Bcl-3 expression is able to block apoptosis in IL-4-deprived cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol BCL3
Entrez
Uniprot