Bcl-xL Antibody

Images

 
Western Blot: Bcl-xL Antibody [NB500-254] - Whole cell lysate (WCL) from 293T cells that were either mock transfected or transfected with an expression construct for Bcl-xL. Antibody used at 0.04 ug/ml.

Product Details

Summary
Product Discontinued
View other related Bcl-xL Primary Antibodies

Order Details


    • Catalog Number
      NB500-254
    • Availability
      Product Discontinued

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Bcl-xL Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residues 1 and 50 of human BCL2-like 1 using the numbering given in SwissProt entry Q07817 (GeneID 598).
Specificity
NB500-254 is specific for human Bcl-xl protein.
Clonality
Polyclonal
Host
Rabbit
Gene
BCL2L1
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:10000-1:25000
Application Notes
Optimal working dilutions should be determined experimentally by the investigator. Prepare working dilution immediately before use. Suggested starting dilutions are as follows. Western Blot: 1:10,000 - 1:25,000 Immunoprecipitation: not tested Histo/Cytochemistry: not tested *In some cases, the antibody may be diluted further than indicated.
Positive Control
Bcl-xL Lysate (NBL1-07946)
Publications
Read Publication using NB500-254.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Bcl-xL Antibody

  • Apoptosis regulator Bcl-X
  • bcl2-L-1
  • BCL2-like 1
  • bcl-2-like protein 1
  • BCLXBCL2LBcl-X
  • BclxL
  • Bcl-xL
  • BCL-XL/S
  • BCLXS
  • bcl-xS
  • DKFZp781P2092

Background

Bcl xL is a 241 amino acid 28 kDa member of Bcl2 family of proteins and an important regulator of apoptosis. Bcl xL forms heterodimers with BAX, BAK, and Bcl2, and its overexpression in tumor cells confers resistance against chemotherapeutic drugs. Bcl xL is phosphorylated on many sites including serine 62, a critical site for Bcl xL response to microtubule damaging drugs such as taxol and vinblastine. Phosphorylation of serine 62 is thought to be mediated by Jun N terminal stress kinase (JNK). Signaling negatively regulates the anti apoptotic function of Bcl xL and controls the growth of neoplastic cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Bcl-xL Antibody (NB500-254)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for Bcl-xL Antibody (NB500-254) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Research Areas for Bcl-xL Antibody (NB500-254)

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Blogs on Bcl-xL.

BAG3 - Hsp70 is my friend!
The BAG proteins are a large family of chaperone regulators governing a wide range of cell processes such as proliferation, survival, stress response, tumorigenesis, neuronal differentiation, growth arrest and apoptosis as reviewed in Takayama et al1....  Read full blog post.

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Bioinformatics

Gene Symbol BCL2L1
Entrez
Uniprot