Bcl-xL Antibody (4A9)

Images

 
Western Blot: Bcl-xL Antibody (4A9) [NBP1-47665] - Analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using antiBCL-XL monoclonal antibody.(1:200)
Immunocytochemistry/ Immunofluorescence: Bcl-Xl Antibody (4A9) [NBP1-47665] - Bcl-Xl Antibody (4A9) Staining of COS7 cells transiently transfected by pCMV6-ENTRY BCL2L1.
Immunohistochemistry-Paraffin: Bcl-xL Antibody (4A9) [NBP1-47665] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-BCL-XL mouse monoclonal antibody.
Western Blot: Bcl-Xl Antibody (4A9) [NBP1-47665] - Bcl-Xl Antibody (4A9) HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BCL2L1(Right lane) cDNA for 48 hrs and lysed. Equivalent ...read more
Western Blot: Bcl-xL Antibody (4A9) [NBP1-47665] - Analysis of extracts (10ug) from 3 different cell lines by using anti-BCL-XL monoclonal antibody.(1:200)
Immunocytochemistry/ Immunofluorescence: Bcl-Xl Antibody (4A9) [NBP1-47665] - Bcl-Xl Antibody (4A9) Immunofluorescent staining of HT29 cells using anti-BCL2L1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Bcl-xL Antibody (4A9) [NBP1-47665] - Staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-BCL-XL mouse monoclonal antibody.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clone
4A9
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

Bcl-xL Antibody (4A9) Summary

Immunogen
Recombinant fragment expressed in E.coli corresponding to amino acids 1-212 of human Bcl-XL
Specificity
This antibody is specific for Homo sapiens BCL2-like 1 (BCL2L1), nuclear gene encoding mitochondrial protein, transcript variant 1.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
BCL2L1
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Purity
Protein A or G purified

Alternate Names for Bcl-xL Antibody (4A9)

  • Apoptosis regulator Bcl-X
  • bcl2-L-1
  • BCL2-like 1
  • bcl-2-like protein 1
  • BCLXBCL2LBcl-X
  • BclxL
  • Bcl-xL
  • BCL-XL/S
  • BCLXS
  • bcl-xS
  • DKFZp781P2092

Background

Bcl-XL belongs to the BCL-2 protein family. BCL-2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. The proteins encoded by this gene are located at the outer mitochondrial membrane, and have been shown to regulate outer mitochondrial membrane channel (VDAC) opening. VDAC regulates mitochondrial membrane potential, and thus controls the production of reactive oxygen species and release of cytochrome C by mitochondria, both of which are the potent inducers of cell apoptosis. Two alternatively spliced transcript variants, which encode distinct isoforms, have been reported. The longer isoform acts as an apoptotic inhibitor and the shorter form acts as an apoptotic activator

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Blogs on Bcl-xL.

BAG3 - Hsp70 is my friend!
The BAG proteins are a large family of chaperone regulators governing a wide range of cell processes such as proliferation, survival, stress response, tumorigenesis, neuronal differentiation, growth arrest and apoptosis as reviewed in Takayama et al1....  Read full blog post.

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Bioinformatics

Gene Symbol BCL2L1
Entrez