Bcl-2 related protein A1 Antibody

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Western Blot: Bcl-2 related protein A1 Antibody [NBP1-52092] - Analysis of Bcl2A1 in Human Bone Marrow lysate (35ug protein in RIPA buffer) using NBP1-52092 at 0.2ug/ml. Primary incubation was 1 hour. Detected by ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

Bcl-2 related protein A1 Antibody Summary

Immunogen
Peptide with sequence C-NVVSVDTART corresponding to internal region according to NP_004040.1, NP_001108207.1.
Epitope
C-NVVSVDTART
Specificity
This antibody is expected to recognize both reported isoforms (NP_004040.1; NP_001108207.1).
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
BCL2A1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 - 0.3 ug/ml
  • Peptide ELISA Detection limit 1:8000
Application Notes
WB: Approx. 20 kDa band observed in human bone marrow lysates (calculated MW of 20.1 kDa band according to NP_004040.1).
Publications
Read Publication using NBP1-52092.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Canine, Bovine, Porcine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Bcl-2 related protein A1 Antibody

  • ACC-1
  • ACC-2
  • Bcl-2 related protein A1
  • BCL2A1
  • Bcl2L5
  • Bcl2-L-5
  • BCL2L5HBPA1
  • Bcl-2-like protein 5
  • BCL2-related protein A1
  • Bfl-1
  • BFL1bcl-2-related protein A1
  • GRS
  • GRSbcl2-L-5
  • hematopoietic BCL2-related protein A1
  • Hemopoietic-specific early response protein
  • Protein BFL-1
  • Protein GRS

Background

Bcl-2 related protein A1; also known as Bcl2A1 and Bfl1, is an anti-apoptotic Bcl-2 family member preferentially expressed in hematopoietic and endothelial cells (1). Bfl1 is involved in a variety of cellular activities such as embryonic development, homeostatis and tumorigenesis. Expression of Bfl1 is stimulated by NF-kB in response to inflammatory stimuli and is up-regulated by TNF-alpha, IL1-beta, IGF1, GMC-SF, CD40 and phorbol ester (2-3). Blf1 has also been shown to reduce the release of pro-apoptotic cytochrome c from the mitochondria, block caspase activation and suppress apoptosis induced by p53 (4-5).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Bcl-2 related protein A1 Antibody (NBP1-52092)(1)

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Bioinformatics

Gene Symbol BCL2A1
Entrez
Uniprot