Human Bcl-2 ELISA Kit (Colorimetric)

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ELISA: Bcl-2 ELISA Kit [NBP1-91188] - Standard curve

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Cell lysate, serum, plasma
Standard Curve Range
0.50 - 32.0 ng/mL
Sensitivity
0.5 ng/mL

Order Details

Human Bcl-2 ELISA Kit (Colorimetric) Summary

Standard Curve Range
0.50 - 32.0 ng/mL
Sensitivity
0.5 ng/mL
Assay Type
Sandwich ELISA
Inter-Assay
12%
Intra-Assay
8.60%
Spike Recovery
71%
Sample Volume
20 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
BCL2

Applications/Dilutions

Application Notes
The human Bcl-2 ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human Bcl-2.

Reactivity Notes

Human

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotin-Conjugate anti-human Bcl-2 monoclonal antibody
  2. Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
  3. Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to human Bcl-2
  4. Adhesive Films
  5. Human Bcl-2 Standard lyophilized, 64 ng/mL upon reconstitution
  6. Lysis Buffer 10x
  7. Sample Diluent
  8. Stop Solution (1M Phosphoric acid)
  9. Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
  10. Streptavidin-HRP
  11. Substrate Solution (tetramethyl-benzidine)

Alternate Names for Human Bcl-2 ELISA Kit (Colorimetric)

  • apoptosis regulator Bcl-2
  • B-cell CLL/lymphoma 2
  • Bcl2
  • Bcl-2

Background

The human Bcl-2 ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human Bcl-2. B-cell lymphoma 2 encoded by a proto-oncogene, is an intracellular membrane-associated protein that functions to block programmed cell death. Expression of Bcl-2 has been reported in a wide range of hematopoietic cells, nonneoplastic epithelia and epithelial malignancies. Bcl-2 levels are low or undetectable in endothelial cells. A decrease in Bcl-2 levels leads to cell death by apoptosis. Overexpression of Bcl-2 on the other hand protects cells from death, but it is neither able to immortalize normal cells, nor to cause tumorgenic transformation of immortalized cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Publications for Bcl-2 ELISA Kit (NBP1-91188) (0)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Bcl-2 ELISA Kit (NBP1-91188). (Showing 1 - 1 of 1 FAQ).

  1. Which BCL-2 isoform is detected with this kit (alpha or beta)? Has any epitope mapping been performed for this kit?
    • The immunogen regions for the 2 antibodies utilized in this Bcl2 ELISA kit are proprietary and cannot be released. This kit does recognizes both the alpha and beta isoform of the Bcl-2 protein.

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Blogs on Bcl-2.

Key Targets in Apoptosis, Necroptosis, and Autophagy
Cell death/recycling pathways such as apoptosis, necroptosis, and autophagy are an integral part of the growth, development, homeostasis as well as the pathophysiology in the life of living organisms. These signaling pathways are highly regulated and...  Read full blog post.

Altered expression of BCL2 in cancer
Similar to other cell processes, the balance between cell survival and cell death is an important equilibrium that when altered expression of genes can lead to a variety of disease.  For example, too little cell death can promote cell overgrowth a...  Read full blog post.

MAPK8/JNK1 - A multifunctional kinase and drug target for cancer therapeutics
The c-Jun N-terminal kinase (JNK) family is a group of regulatory kinases with important functions in cell morphogenesis, inflammation, differentiation, and cell death (1). Aberrant activation of JNK family proteins in cancers has led to interest i...  Read full blog post.

p53 - Investigating an important tumor suppressor
p53 is a tumor suppressor that has a central role in regulating cell cycle arrest, DNA repair, and apoptosis. p53 is widely studied for its role in cancer and is mutated or altered in more than half of all cancers (1). This widespread role in tumor...  Read full blog post.

Bcl-2 - an antiapoptotic protein with an important role in cancer cell survival
B-cell lymphoma 2 (Bcl-2) protein is an oncogene that normally acts as an apoptotic inhibitor and localizes to the mitochondrial membrane where it prevents the release of cytochrome c. The Bcl-2 protein family consists of over 20 proteins each co...  Read full blog post.

Beclin 1 - a key regulator of autophagosome formation
The Beclin 1 protein is a central regulator of autophagy in mammalian cells. Autophagy is an essential process used to maintain cellular homeostasis by degrading and recycling cellular components such as damaged or worn out organelles and macromole...  Read full blog post.

BNIP3 - a regulator of mitochondrial autophagy and cell death
Bcl-2 nineteen-kilodalton interacting protein 3 (BNIP3) is a pro-apoptotic BH3-only protein. BNIP3 localizes to the mitochondrial membrane where it plays a key role in mitochondrial autophagy and cell death pathways. Similar to other Bcl-2 family m...  Read full blog post.

BAG3 - Hsp70 is my friend!
The BAG proteins are a large family of chaperone regulators governing a wide range of cell processes such as proliferation, survival, stress response, tumorigenesis, neuronal differentiation, growth arrest and apoptosis as reviewed in Takayama et al1....  Read full blog post.

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Bioinformatics

Gene Symbol BCL2
Entrez