Bcl-2 Antibody


Western Blot: Bcl2 Antibody [NB300-900] - (1ug/ml) of Human Lymph Node lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Product Details

Reactivity HuSpecies Glossary
Applications WB, PEP-ELISA
0.5 mg/ml

Order Details

Bcl-2 Antibody Summary

Peptide with sequence AGRTGYDNREIVMKYC corresponding to N-Terminus according to NP_000648.2.
This antibody is expected to recognise both reported isoforms (NP_000624; NP_000648)
Immunogen affinity purified
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Packaging, Storage & Formulations

Store at -20C. Avoid freeze-thaw cycles.
Tris saline, pH 7.3 with 0.5% BSA.
0.02% Sodium Azide
0.5 mg/ml
Immunogen affinity purified


Application Notes
WB: Approx. 27+25 kDa band observed in human Lymph lysates (calculated MW of 26.3 kDa band according to NP_000624.2 and of 22.2 kDa band according to NP_000648.2).
Read Publications using NB300-900.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Mouse, Rat, Canine.

Alternate Names for Bcl-2 Antibody

  • apoptosis regulator Bcl-2
  • B-cell CLL/lymphoma 2
  • Bcl2
  • Bcl-2


This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Two transcript variants, produced by alternate splicing, differ in their C-terminal ends.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Bcl-2 Antibody (NB300-900)(4)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies


Isotype Controls

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Blogs on Bcl-2.

MAPK8/JNK1 - A multifunctional kinase and drug target for cancer therapeutics
The c-Jun N-terminal kinase (JNK) family is a group of regulatory kinases with important functions in cell morphogenesis, inflammation, differentiation, and cell death (1). Aberrant activation of JNK family proteins in cancers has led to interest i...  Read full blog post.

p53 - Investigating an important tumor suppressor
p53 is a tumor suppressor that has a central role in regulating cell cycle arrest, DNA repair, and apoptosis. p53 is widely studied for its role in cancer and is mutated or altered in more than half of all cancers (1). This widespread role in tumor...  Read full blog post.

Bcl-2 - an antiapoptotic protein with an important role in cancer cell survival
B-cell lymphoma 2 (Bcl-2) protein is an oncogene that normally acts as an apoptotic inhibitor and localizes to the mitochondrial membrane where it prevents the release of cytochrome c. The Bcl-2 protein family consists of over 20 proteins each co...  Read full blog post.

Beclin 1 - a key regulator of autophagosome formation
The Beclin 1 protein is a central regulator of autophagy in mammalian cells. Autophagy is an essential process used to maintain cellular homeostasis by degrading and recycling cellular components such as damaged or worn out organelles and macromole...  Read full blog post.

BNIP3 - a regulator of mitochondrial autophagy and cell death
Bcl-2 nineteen-kilodalton interacting protein 3 (BNIP3) is a pro-apoptotic BH3-only protein. BNIP3 localizes to the mitochondrial membrane where it plays a key role in mitochondrial autophagy and cell death pathways. Similar to other Bcl-2 family m...  Read full blog post.

BAG3 - Hsp70 is my friend!
The BAG proteins are a large family of chaperone regulators governing a wide range of cell processes such as proliferation, survival, stress response, tumorigenesis, neuronal differentiation, growth arrest and apoptosis as reviewed in Takayama et al1....  Read full blog post.

Contact Information

Product PDFs


Gene Symbol BCL2

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