BCKDK Antibody (11C9)

Images

 
Western Blot: BCKDK Antibody (11C9) [NBP1-47664] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BCKDK (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates ...read more
Immunocytochemistry/ Immunofluorescence: BCKDK Antibody (11C9) [NBP1-47664] Staining of COS7 cells transiently transfected by pCMV6-ENTRY BCKDK.
Immunohistochemistry-Paraffin: BCKDK Antibody (11C9) [NBP1-47664] - Staining of paraffin-embedded Human Ovary tissue using anti-BCKDK mouse monoclonal antibody.
Immunohistochemistry-Paraffin: BCKDK Antibody (11C9) [NBP1-47664] - Staining of paraffin-embedded Human endometrium tissue using anti-BCKDK mouse monoclonal antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clone
11C9
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

BCKDK Antibody (11C9) Summary

Immunogen
Protein expressed in 293T cell transfected with human BCKDK expression vector
Specificity
This antibody is specific for Homo sapiens branched chain ketoacid dehydrogenase kinase (BCKDK), nuclear gene encoding mitochondrial protein, transcript variant 1.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
BCKDK
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50

Reactivity Notes

Human

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for BCKDK Antibody (11C9)

  • BCKDHKIN
  • branched chain alpha-ketoacid dehydrogenase kinase
  • branched chain ketoacid dehydrogenase kinase
  • EC 2.7.11
  • EC 2.7.11.4
  • mitochondrial

Background

Catalyzes the phosphorylation and inactivation of the branched-chain alpha-ketoacid dehydrogenase complex,the key regulatory enzyme of the valine, leucine and isoleucine catabolic pathways. Key enzyme that regulate the activity state of the BCKD complex.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol BCKDK
Entrez