BCAT1 Antibody (3F5)

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Western Blot: BCAT1 Antibody (3F5) [NBP2-01826] - Analysis of extracts (10ug) from a mouse cell line and a mouse tissue by using anti-BCAT1 monoclonal antibody. (1:200)
Immunohistochemistry-Paraffin: BCAT1 Antibody (3F5) [NBP2-01826] - Staining of paraffin-embedded Human tonsil using anti-BCAT1 mouse monoclonal antibody.
Western Blot: BCAT1 Antibody (3F5) [NBP2-01826] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BCAT1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates ...read more
Western Blot: BCAT1 Antibody (3F5) [NBP2-01826] Analysis of extracts (35ug) from 9 different cell lines by using anti-BCAT1 monoclonal antibody.
Western Blot: BCAT1 Antibody (3F5) [NBP2-01826] - Analysis of extracts (10ug) from 9 Human tissue by using anti-BCAT1 monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: ...read more
Immunohistochemistry-Paraffin: BCAT1 Antibody (3F5) [NBP2-01826] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-BCAT1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: BCAT1 Antibody (3F5) [NBP2-01826] - Staining of paraffin-embedded Carcinoma of Human liver tissue using anti-BCAT1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: BCAT1 Antibody (3F5) [NBP2-01826] - Staining of paraffin-embedded Human lymphoma tissue using anti-BCAT1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: BCAT1 Antibody (3F5) [NBP2-01826] - Staining of paraffin-embedded Human pancreas tissue using anti-BCAT1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: BCAT1 Antibody (3F5) [NBP2-01826] - Staining of paraffin-embedded Human prostate tissue using anti-BCAT1 mouse monoclonal antibody.

Product Details

Summary
Reactivity Hu, Mu, CaSpecies Glossary
Applications WB, IHC, IHC-P
Clone
3F5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

BCAT1 Antibody (3F5) Summary

Immunogen
Full length human recombinant protein of human BCAT1(NP_005495) produced in HEK293T cell.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
BCAT1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-2000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:150

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for BCAT1 Antibody (3F5)

  • BCAT(c)
  • BCAT1
  • BCAT-1
  • BCATC
  • BCT1
  • BCT1PNAS121
  • branched chain amino-acid transaminase 1, cytosolic
  • branched chain aminotransferase 1, cytosolic
  • branched-chain-amino-acid aminotransferase, cytosolic
  • DKFZp686E12175
  • EC 2.6.1.42
  • ECA39
  • MECA39
  • placental protein 18
  • PNAS121
  • PP18
  • Protein ECA39

Background

This gene encodes the cytosolic form of the enzyme branched-chain amino acid transaminase. This enzyme catalyzes the reversible transamination of branched-chain alpha-keto acids to branched-chain L-amino acids essential for cell growth. Two different clinical disorders have been attributed to a defect of branched-chain amino acid transamination: hypervalinemia and hyperleucine-isoleucinemia. As there is also a gene encoding a mitochondrial form of this enzyme, mutations in either gene may contribute to these disorders. Alternatively spliced transcript variants have been described. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol BCAT1
Entrez