Bax Antibody


Western Blot: Bax Antibody [NBP1-77151] - Analysis of Bax in HL-60 cell lysates with anti-Bax (NT) using this antibody at (A) 1, (B) 2, and (C) 4 ug/ml.
Immunocytochemistry/ Immunofluorescence: Bax Antibody [NBP1-77151] - HL60 cells with Bax antibody at 2 ug/mL.
Immunohistochemistry: Bax Antibody [NBP1-77151] - Staining of HL-60 cells at 2 ug/mL.

Product Details

Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF, ICC
1 mg/ml

Order Details

Bax Antibody Summary

Antibody was raised against a peptide corresponding to 16 amino acids near the amino-terminus of human Bax.
Ion exchange chromatography
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Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.02% Sodium Azide
1 mg/ml
Ion exchange chromatography


  • Western Blot 1-4ug/ml
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 2ug/ml
  • Immunocytochemistry
Application Notes
Bax antibody can be used for detection of Bax by Western blot at 2 mg/ml.
Control Peptide
Bax Peptide (NBP1-77151PEP)

Reactivity Notes


Alternate Names for Bax Antibody

  • apoptosis regulator BAX
  • BCL2-associated X protein
  • Bcl2-L-4
  • BCL2L4bcl2-L-4
  • Bcl-2-like protein 4


Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells. Disruption of this process has been implicated in a variety of diseases such as cancer. The Bcl-2 family of proteins is comprised of critical regulators of apoptosis that can be divided into two classes: those that inhibit apoptosis and those that promote cell death. Bax, a pro-apoptotic Bcl-2 family member, is a cytosolic protein that changes conformation and translocates to the mitochondria following apoptotic stimuli. It is thought to share significant functional homology with Bak, another pro-apoptotic Bcl-2 family member, as disruption of bax or bak has little effect on cell death, but mice lacking both genes display multiple developmental defects and cells lacking both show decreased apoptotic capability.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies


Isotype Controls

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Blogs on Bax.

p53 - Investigating an important tumor suppressor
p53 is a tumor suppressor that has a central role in regulating cell cycle arrest, DNA repair, and apoptosis. p53 is widely studied for its role in cancer and is mutated or altered in more than half of all cancers (1). This widespread role in tumor...  Read full blog post.

UVRAG - A regulator of membrane trafficking in autophagy and endocytosis
UV resistance-associated gene (UVRAG) is a tumor suppressor that is commonly mutated in colon and breast cancer. While UVRAG was discovered for its ability to complement UV sensitivity in xeroderma pigmentosum cells, its main functions are in auto...  Read full blog post.

Caspase 9: The Suicidal Cell Whisperer
Cell death via apoptosis is a key cellular function triggered by the cell death receptor family and their ligands which signal through downstream adaptor molecules and the caspase protease family. Among the subclass of initiator caspases that include...  Read full blog post.

Dynamin-related Protein 1 (DRP1) in Mitochondria and Apoptosis.
Dynamin-related Protein 1 (DRP1) is known to function in mitochondrial and peroxisomal division and mediate membrane fission through oligomerization into ring-like structure and sever the mitochondrial membrane, through a GTP hydrolysis-dependent mech...  Read full blog post.

Ku70's Roles in Double Strand and Mismatch DNA Repair
Ku70 is a 70 kDa protein that was shown to be involved in multiple cellular pathways, mainly involving DNA repair and recombination. Among these are the non-homologus end repairs of DNA double strand breaks. Ku70 was first identified as an autoantigen...  Read full blog post.

Bax Research Gives New Insight into Oxidative Apoptosis
Bax is a member of the Bcl-2 family; an extensive range of proteins which play key roles in apoptosis, or programmed cell death, by regulating outer mitochondrial membrane permeability. We at Novus Biologicals are one of the leading antibody suppliers...  Read full blog post.

Contact Information

Product PDFs


Gene Symbol BAX

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