BARD1 Antibody

Images

 
Western Blot: BARD1 Antibody [NBP1-19636] - Analysis of BARD1 (R8) pAb at 1:500 dilution Lane1:Hela cell lysate Lane2:Raw264.7 cell lysate Lane3:H9C2 cell lysate
Western Blot: BARD1 Antibody [NBP1-19636] - extracts from HeLa cells.
Western Blot: BARD1 Antibody [NBP1-19636] Extracts from HeLa cells.

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP1-19636
    • Availability
      Product Discontinued

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BARD1 Antibody Summary

Immunogen
Synthetic peptide, corresponding to the N-terminual of Human BARD1.
Clonality
Polyclonal
Host
Rabbit
Gene
BARD1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Paraffin 1:50-1:200
Theoretical MW
87 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NBP1-19636 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2)
Preservative
15mM Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for BARD1 Antibody

  • BARD-1
  • BRCA1 associated RING domain 1
  • BRCA1-associated RING domain gene 1
  • BRCA1-associated RING domain protein 1
  • EC 6.3.2.-

Background

Mutations within the BRCA1 gene, localized to chromosome 17q, are believed to account for approximately 45% of families with increased incidence of both early-onset breast cancer and ovarian cancer. The BRCA1 gene is expressed in numerous tissues, including breast and ovary, and encodes a predicted protein of 1863 amino acids. This protein contains a RING domain near the N-terminus and appears to encode a tumor suppressor. BARD1 (BRCA1-associated RING domain protein 1) and BAP1 (BRCA1-associated protein 1) have both been shown to bind to the N-terminus of BRCA1 and are potential mediators of tumor suppression. BARD1 contains an N-terminal RING domain and three tandem ankyrin repeats. The C-terminus of BARD1 contains a region with sequence homology to BRCA1, termed the BRCT domain. BAP1 is an ubiquitin hydrolase and has been shown to enhance BRCA1-mediated cell growth suppression.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for BARD1 Antibody (NBP1-19636)(3)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol BARD1
Entrez
Uniprot