BAMBI/NMA Knockout 293T Cell Lysate Summary
Preparation Method |
Knockout achieved by using CRISPR/Cas9,1 bp insertion in exon 1 |
Gene |
BAMBI |
Applications/Dilutions
Application Notes |
You will receive 1 vial (100ug) of knockout cell lysate and 1 vial (100ug) of Parental cell lysate. Lysate can be diluted with 1X SDS sample buffer and will be stable at -20 degrees C for 12 months. Minimize freeze-thaw cycles. |
Packaging, Storage & Formulations
Storage |
Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles. |
Buffer |
0.1 mg cell homogenate lyophilized in RIPA buffer made with double-knockout cell lines. |
Concentration |
LYOPH |
Reconstitution Instructions |
To use as WB negative control, spin down briefly and resuspend in 100 uL 1xSDS sample buffer (2% SDS, 60 mM Tris-HCl pH 6.8, 10% Glycerol, 0.02% Bromophenol blue, 60 mM beta-mercaptoethanol). Boil the lysate for 3 - 5 minutes before loading it onto gel. |
Lysate Details for Array
Type |
Knockout 293T Cell |
Life Stage |
Embryonic |
Tissue |
Kidney |
Notes
Powered by EDIGENE.
Validation of antibody specificity is critical and verification of antibody performance against knockout samples is one way to guarantee that an antibody recognizes a specific target. Novus' KO cell lysate can be used as a negative control for western blots and to confirm the specificity of antibodies.
Alternate Names for BAMBI/NMA Knockout 293T Cell Lysate
Background
This gene encodes a transmembrane glycoprotein related to the type I receptors of the transforming growth factor-beta (TGF-beta) family, whose members play important roles in signal transduction in many developmental and pathological processes. The encoded protein however is a pseudoreceptor, lacking an intracellular serine/threonine kinase domain required for signaling. Similar proteins in frog, mouse and zebrafish function as negative regulators of TGF-beta, which has led to the suggestion that the encoded protein may function to limit the signaling range of the TGF-beta family during early embryogenesis. [provided by RefSeq, Jul 2008]
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are
guaranteed for 6 months from date of receipt.
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