BAK Antibody (8B4) [DyLight 405]

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Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA, ICC/IF
Clone
8B4
Clonality
Monoclonal
Host
Mouse

BAK Antibody (8B4) [DyLight 405] Summary

Immunogen
Recombinant human BAK1 (29-187aa) purified from E.coli
Specificity
Anti-human BAK1 mAb, clone 8B4, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human BAK1 protein.
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
BAK1
Purity
Protein G purified
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Test in a species/application not listed above to receive a full credit towards a future purchase.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Conjugate
DyLight 405
Format
DyLight 405 (A=400, E=420)
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Applications/Dilutions

Dilutions
  • Western Blot
  • ELISA
  • Immunocytochemistry/Immunofluorescence
Application Notes
The antibody has been tested by ELISA, Western blot and Immunofluorescence analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis and Immunofluorescence is 1:250 ~ 500. Recommended starting dilution is 1:250

Reactivity Notes

Human, Mouse. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Alternate Names for BAK Antibody (8B4) [DyLight 405]

  • Apoptosis regulator BAK
  • BAK
  • BAK1
  • BAK-LIKE
  • BAKMGC3887
  • BCL2-antagonist/killer 1
  • BCL2L7
  • bcl2-L-7
  • BCL2L7bcl-2 homologous antagonist/killer
  • BCL2-like 7 protein
  • Bcl-2-like protein 7
  • CDN1
  • MGC117255
  • pro-apoptotic protein BAK

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for BAK Antibody (NBP1-74026V) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Allophycocyanin Labeled NBP1-74026APC
DyLight 350 Labeled NBP1-74026UV
DyLight 405LS Labeled NBP1-74026V3
DyLight 488 Labeled NBP1-74026G
DyLight 680 Labeled NBP1-74026FR
DyLight 755 Labeled NBP1-74026IR
PE Labeled NBP1-74026PE
PerCP Labeled NBP1-74026PCP

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Blogs on BAK.

IRE1 alpha dependent apoptotic-signaling pathway
Despite in depth characterization of the role of IRE1 alpha (inositol-requiring enzyme 1 alpha) in activating the unfolded protein response (UPR) in the ER - little is known about the molecular mechanisms by which this ER protein has shown to reg...  Read full blog post.

p53 - Investigating an important tumor suppressor
p53 is a tumor suppressor that has a central role in regulating cell cycle arrest, DNA repair, and apoptosis. p53 is widely studied for its role in cancer and is mutated or altered in more than half of all cancers (1). This widespread role in tumor...  Read full blog post.

Dynamin-related Protein 1 (DRP1) in Mitochondria and Apoptosis.
Dynamin-related Protein 1 (DRP1) is known to function in mitochondrial and peroxisomal division and mediate membrane fission through oligomerization into ring-like structure and sever the mitochondrial membrane, through a GTP hydrolysis-dependent mech...  Read full blog post.

Contact Information

Product PDFs

Bioinformatics

Gene Symbol BAK1
Entrez
Uniprot

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