BAK Antibody (8B4)

Images

 
Western Blot: BAK Antibody (8B4) [NBP1-74026] - The cell lysate (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human BAK1 antibody (1:1000). Proteins were visualized using a goat ...read more
Immunocytochemistry/ Immunofluorescence: BAK Antibody (8B4) [NBP1-74026] - Analysis of BAK1 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human HeLa antibody (1:100) with goat ...read more
Flow Cytometry: BAK Antibody (8B4) [NBP1-74026] - Analysis of BAK1 in HeLa cell line, staining at 2-5ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF
Clone
8B4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

BAK Antibody (8B4) Summary

Immunogen
Recombinant human BAK1 (29-187aa) purified from E.coli
Specificity
Anti-human BAK1 mAb, clone 8B4, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human BAK1 protein.
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
BAK1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500
  • ELISA 1:100-1:2000
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence 1:500
Application Notes
The antibody has been tested by ELISA, Western blot and Immunofluorescence analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis and Immunofluorescence is 1:250 ~ 500. Recommended starting dilution is 1:250

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4) and 10% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for BAK Antibody (8B4)

  • Apoptosis regulator BAK
  • BAK
  • BAK1
  • BAK-LIKE
  • BAKMGC3887
  • BCL2-antagonist/killer 1
  • BCL2L7
  • bcl2-L-7
  • BCL2L7bcl-2 homologous antagonist/killer
  • BCL2-like 7 protein
  • Bcl-2-like protein 7
  • CDN1
  • MGC117255
  • pro-apoptotic protein BAK

Background

BAK1 encodes an receptor-like kinase (RLK) with a putative extracellular domain, a single transmembrane domain, an intracellular-juxtamembrane domain, and a kinase domain. BAK1 expression is associated with the progression of Prostate cancer (CaP). BAK1 appears to function in distinct receptor-signaling complexes to integrate multiple microbe-associated molecular pattern (MAMP) perception into downstream-signaling events. BAK1 can serve multiple purposes to simultaneously modulate MAMP-receptor complexes, BR signaling, and cell death for the benefit of bacterial infection, life style, and fitness.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for BAK Antibody (NBP1-74026) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP1-74026AF405
Alexa Fluor 488 Labeled NBP1-74026AF488
Alexa Fluor 647 Labeled NBP1-74026AF647
Alexa Fluor 700 Labeled NBP1-74026AF700
DyLight 350 Labeled NBP1-74026UV
DyLight 405 Labeled NBP1-74026V
DyLight 488 Labeled NBP1-74026G
DyLight 680 Labeled NBP1-74026FR
DyLight 755 Labeled NBP1-74026IR
PE Labeled NBP1-74026PE

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p53 - Investigating an important tumor suppressor
p53 is a tumor suppressor that has a central role in regulating cell cycle arrest, DNA repair, and apoptosis. p53 is widely studied for its role in cancer and is mutated or altered in more than half of all cancers (1). This widespread role in tumor...  Read full blog post.

Dynamin-related Protein 1 (DRP1) in Mitochondria and Apoptosis.
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Bioinformatics

Gene Symbol BAK1
Entrez
Uniprot