BAK Antibody (8B4)


Western Blot: Bak Antibody (AT8B4) [NBP1-74026] - PAGE, transferred to NC membrane and probed with anti-human BAK1 (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an more
Immunocytochemistry/ Immunofluorescence: Bak Antibody (AT8B4) [NBP1-74026] - Immunofluorescence of human HeLa cells stained with Hoechst 3342 (Blue) for nucleus staining and monoclonal anti-human BAK1 antibody (1:500) more

Product Details

Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA, ICC/IF
1 mg/ml

BAK Antibody (8B4) Summary

Recombinant human BAK1 (29-187aa) purified from E.coli
Anti-human BAK1 mAb, clone 8B4, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human BAK1 protein.
IgG2a Kappa
Protein G purified
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Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Liquid. In Phosphate-Buffered Saline (pH 7.4)
0.1% Sodium Azide
1 mg/ml
Protein G purified


  • Western Blot 1:500
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:500
Application Notes
The antibody has been tested by ELISA, Western blot and Immunofluorescence analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis and Immunofluorescence is 1:250 ~ 500. Recommended starting dilution is 1:250

Reactivity Notes

Human, Mouse. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Alternate Names for BAK Antibody (8B4)

  • Apoptosis regulator BAK
  • BAK1
  • BAKMGC3887
  • BCL2-antagonist/killer 1
  • BCL2L7
  • bcl2-L-7
  • BCL2L7bcl-2 homologous antagonist/killer
  • BCL2-like 7 protein
  • Bcl-2-like protein 7
  • CDN1
  • MGC117255
  • pro-apoptotic protein BAK


BAK1 encodes an receptor-like kinase (RLK) with a putative extracellular domain, a single transmembrane domain, an intracellular-juxtamembrane domain, and a kinase domain. BAK1 expression is associated with the progression of Prostate cancer (CaP). BAK1 appears to function in distinct receptor-signaling complexes to integrate multiple microbe-associated molecular pattern (MAMP) perception into downstream-signaling events. BAK1 can serve multiple purposes to simultaneously modulate MAMP-receptor complexes, BR signaling, and cell death for the benefit of bacterial infection, life style, and fitness.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for BAK Antibody (NBP1-74026) (0)

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Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor (R) 405 Labeled NBP1-74026AF405
Alexa Fluor (R) 488 Labeled NBP1-74026AF488
Alexa Fluor (R) 647 Labeled NBP1-74026AF647
Alexa Fluor (R) 700 Labeled NBP1-74026AF700
Allophycocyanin Labeled NBP1-74026APC
DyLight 350 Labeled NBP1-74026UV
DyLight 405 Labeled NBP1-74026V
DyLight 405LS Labeled NBP1-74026V3
DyLight 488 Labeled NBP1-74026G
DyLight 680 Labeled NBP1-74026FR
DyLight 755 Labeled NBP1-74026IR
PE Labeled NBP1-74026PE
PerCP Labeled NBP1-74026PCP

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Despite in depth characterization of the role of IRE1 alpha (inositol-requiring enzyme 1 alpha) in activating the unfolded protein response (UPR) in the ER - little is known about the molecular mechanisms by which this ER protein has shown to reg...  Read full blog post.

p53 - Investigating an important tumor suppressor
p53 is a tumor suppressor that has a central role in regulating cell cycle arrest, DNA repair, and apoptosis. p53 is widely studied for its role in cancer and is mutated or altered in more than half of all cancers (1). This widespread role in tumor...  Read full blog post.

Dynamin-related Protein 1 (DRP1) in Mitochondria and Apoptosis.
Dynamin-related Protein 1 (DRP1) is known to function in mitochondrial and peroxisomal division and mediate membrane fission through oligomerization into ring-like structure and sever the mitochondrial membrane, through a GTP hydrolysis-dependent mech...  Read full blog post.

Contact Information

Product PDFs


Gene Symbol BAK1

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