BAG5 Antibody

Images

 
Western Blot: BAG5 Antibody [NBP1-33300] - Mouse tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with BAG5 antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody ...read more
Immunocytochemistry/ Immunofluorescence: BAG5 Antibody [NBP1-33300] - Paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.
Western Blot: BAG5 Antibody [NBP1-33300] - Sample (30 ug of whole cell lysate) A: Hela B: Molt-4 10% SDS PAGE; antibody diluted at 1:1000.

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, PmSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

BAG5 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human BAG5. The exact sequence is proprietary.
Predicted Species
Rat (91%), Bovine (93%), Primate (99%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
BAG5
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
Theoretical MW
51 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Chimpanzee (99%), Chicken (81%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 10% Glycerol
Preservative
0.01% Thimerosal
Purity
Immunogen affinity purified

Alternate Names for BAG5 Antibody

  • BAG family molecular chaperone regulator 5
  • BAG-5
  • BAG-family molecular chaperone regulator-5
  • Bcl-2-associated athanogene 5
  • BCL2-associated athanogene 5
  • KIAA0873

Background

The protein encoded by this gene is a member of the BAG1-related protein family. BAG1 is an anti-apoptotic protein that functions through interactions with a variety of cell apoptosis and growth related proteins including BCL-2, Raf-protein kinase, steroid hormone receptors, growth factor receptors and members of the heat shock protein 70 kDa family. This protein contains a BAG domain near the C-terminus, which could bind and inhibit the chaperone activity of Hsc70/Hsp70. Three transcript variants encoding two different isoforms have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Discover related pathways, diseases and genes to BAG5 Antibody (NBP1-33300). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Blogs on BAG5.

BAG3 - Hsp70 is my friend!
The BAG proteins are a large family of chaperone regulators governing a wide range of cell processes such as proliferation, survival, stress response, tumorigenesis, neuronal differentiation, growth arrest and apoptosis as reviewed in Takayama et al1....  Read full blog post.

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Bioinformatics

Gene Symbol BAG5