Bag-1 Antibody

Images

 
Western Blot: Bag-1 Antibody [NBP1-32124] - Sample (30 ug of whole cell lysate) A: H1299 10% SDS PAGE Bag1 antibody, antibody diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: Bag-1 Antibody [NBP1-32124] - Paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.

Product Details

Summary
Reactivity Hu, BvSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

Bag-1 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human Bag1. The exact sequence is proprietary.
Localization
Isoform 2: Cytoplasm, Nucleus
Predicted Species
Bovine (91%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
BAG1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
Theoretical MW
39 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Expected cross reactivity based on sequence homology: Mouse (82%) and Rat (81%), Pig (87%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 10% Glycerol
Preservative
0.01% Thimerosal
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Bag-1 Antibody

  • BAG family molecular chaperone regulator 1
  • Bag1
  • Bag-1
  • Bcl-2 associating athanogene-1 protein
  • Bcl-2-associated athanogene 1
  • BCL2-associated athanogene
  • Bcl-2-binding protein
  • glucocortoid receptor-associated protein RAP46,46-KD
  • HAP
  • RAP46

Background

Bag1 is a multifunctional protein that blocks apoptosis and interacts with several types of proteins. These include the Bcl 2 family proteins, the kinase Raf 1, certain tyrosine kinase growth factor receptors, and steroid hormone receptors, possibly by virtue of its ability to regulate the Hsp70 / Hsc70 family of molecular chaperones. Bag1 binds the ATPase domains of Hsp70 and Hsc70, modulating their chaperone activity and functioning as a competitive antagonist of the co chaperone Hip. Human BAG 1 (hBAG 1) cDNA is identical to a hormone receptor binding protein RAP46. Human Bag1 protein displays different isoforms of 36 kDa, 46 kDa, and 50 kDa.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Blogs on Bag-1.

BAG3 - Hsp70 is my friend!
The BAG proteins are a large family of chaperone regulators governing a wide range of cell processes such as proliferation, survival, stress response, tumorigenesis, neuronal differentiation, growth arrest and apoptosis as reviewed in Takayama et al1....  Read full blog post.

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Bioinformatics

Gene Symbol BAG1
Entrez