BAFFR/TNFRSF13C Antibody (204406) [Unconjugated]

Images

 
Mouse splenocytes were stained with Rat Anti-Mouse B220/CD45R APC‑conjugated Monoclonal Antibody (Catalog # FAB1217A) and either (A) Rat Anti-Mouse BAFF R/TNFRSF13C Monoclonal Antibody (Catalog # MAB1755) or (B) Rat ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Flow, CyTOF-ready
Clone
204406
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
LYOPH

BAFFR/TNFRSF13C Antibody (204406) [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse BAFF R/TNFRSF13C
Gly2-Ala71
Accession # Q9D8D0
Specificity
Detects mouse BAFF R/TNFRSF13C in direct ELISAs. In direct ELISAs, 100% cross-reactivity with recombinant human (rh) BAFF R is observed, approximately 10‑25% cross-reactivity with recombinant mouse (rm) 4‑1BB, rmDR3, rhDR6, rmEDAR, rhHVEM, rmNGF R, rmOX40, and rmTROY is observed, and no cross-reactivity with rmCD27, rmCD30, rmCD40, rmFAS, rmGITR, rmLymphotoxin  beta R, rmOPG, rmRANK, rmTNFRI, or rmTNF RII is observed.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Rat
Gene
TNFRSF13C
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 0.25 ug/10^6 cells
  • CyTOF-ready
Publications
Read Publications using
MAB1755 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for BAFFR/TNFRSF13C Antibody (204406) [Unconjugated]

  • BAFF R
  • BAFFR
  • BR3
  • CD268
  • TNFRSF13C

Background

B-cell activating factor (BAFF), also known as BlyS, TALL-1, TNAK, and zTNF4, is a TNF ligand superfamily member and has been designated TNFSF13B. It is produced by macrophages, dendritic cells, and T lymphocytes. BAFF promotes the survival of B cells and is essential for B cell maturation (1‑4). BAFF binds to three TNF receptor superfamily members: B‑cell maturation antigen (BCMA/TNFRSF17), transmembrane activator and calcium-modulator and cyclophilin ligand interactor (TACI/TNFRSF13B) and BAFF receptor (BAFF R/BR3/TNFRSF 13C). These receptors are type III transmembrane proteins that lack a signal peptide. Whereas TACI and BCMA bind BAFF and another TNF superfamily ligand, APRIL(a proliferation-inducing ligand), BAFF-R selectively binds BAFF. Mouse BAFF-R cDNA encodes a 175 amino acid residue (aa) transmembrane protein with a 71 aa extracellular domain, a 21 aa transmembrane domain, and a 83 aa cytoplasmic region. A second isoform of BAFF R that has a 72 aa cytoplamic region can also be produced by alternative splicing. The BAFF R extracellular domain lacks the TNF receptor canonical cysteine-rich domain (CRD) and contains only a partial CRD with four cysteine residues. Human and mouse BAFF R share 56% aa sequence identity. BAFF R is highly expressed in spleen, lymph node and resting B cells. It is also expressed at lower levels in activated B cells, in resting CD4+ T cells, in thymus and peripheral blood leukocytes. BAFF knockout mice lack mature B cells and has profound defects in antibody mediated immune responses. Similarly, A/WySnJ mice that are defective in BAFF R intracellular signaling also lack mature B cells, suggesting that BAFF R is the critical receptor for BAFF during B lymphopoiesis. In contrast, BCMA- or TACI-deficient mice have no major defect in B‑cell development. While the function of BCMA is not defined, TACI has been shown to control
B-cell homeostasis and T-cell-independent immune responses.

  1. Rolink, A.G. and F. Melcher (2002) Curr. Opin. Immunol. 14:266.
  2. Mackay, F. and J.L. Browning (2002) Nature Reviews Immunology 2:464.
  3. Laabi, Y. et al. (2001) Current Biol. 11:R1013.
  4. Thompson, J.S. et al. (2001) Science 14:2108.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for BAFFR/TNFRSF13C Antibody (MAB1755)(3)

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Bioinformatics

Gene Symbol TNFRSF13C
Uniprot