BAFF R/TNFRSF13C Antibody [Unconjugated]

Images

 
Recombinant Human BAFF R/TNFRSF13C Fc Chimera (Catalog #1162‑BR) inhibits Recombinant Human BAFF/BLyS/TNFSF13B (Catalog # 2149‑BF) induced proliferation in mouse B cells in a dose-dependent manner (orange line). ...read more
Peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD19 Fluorescein‑conjugated Monoclonal Antibody (Catalog # FAB4867F) and either (A) Goat Anti-Human BAFF R/TNFRSF13C Antigen ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, B/N, CyTOF-ready
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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Catalog# & Formulation Size Price

BAFF R/TNFRSF13C Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human BAFF R/TNFRSF13C
Arg2-Ala71
Accession # Q96RJ3
Specificity
Detects human BAFF R in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross-reactivity with recombinant mouse BAFF R and less than 2% cross-reactivity with recombinant human (rh) 4-1BB, rhCD27, rhCD30, rhCD40, rhDR3, rhDR6, rhEDAR, rhFas, rhGITR, rhHVEM, rhNGF R, rhOPG, rhRANK, rhTNF RI, and rhTNF RII is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
TNFRSF13C
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
  • Flow Cytometry 0.25 ug/10^6 cells
  • CyTOF-ready
  • Neutralization 3-15 ug/mL
Publications
Read Publications using
AF1162 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for BAFF R/TNFRSF13C Antibody [Unconjugated]

  • BAFF R
  • BAFFR
  • BR3
  • CD268
  • TNFRSF13C

Background

B cell activating factor (BAFF), also known as BlyS, TALL-1, TNAK, and zTNF4, is a TNF ligand superfamily member and has been designated TNFSF13B. Produced by macrophages, dendritic cells, and T lymphocytes, BAFF promotes the survival of B cells and is essential for B cell maturation (1-4). BAFF binds to three TNF receptor superfamily members: B cell maturation antigen (BCMA/TNFRSF17), transmembrane activator and calcium-modulator and cyclophilin ligand interactor (TACI/TNFRSF13B) and BAFF receptor (BAFF R/BR3/TNFRSF13C). These receptors are type III transmembrane proteins that lack a signal peptide. Whereas TACI and BCMA bind BAFF and another TNF superfamily ligand, APRIL (a proliferation-inducing ligand), BAFF R selectively binds BAFF. The BAFF R extracellular domain lacks the TNF receptor canonical cysteine-rich domain (CRD) and contains only a partial CRD with four cysteine residues. Human and mouse BAFF R share 56% aa sequence identity. BAFF R is highly expressed in spleen, lymph node and resting B cells. It is also expressed at lower levels in activated B cell, in resting CD4+ T cells, in thymus and peripheral blood leukocytes. BAFF knockout mice lack mature B cells. Similarly, A/WySnJ mice that are defective in BAFF-R intracellular signaling also lack mature B cells, suggesting that BAFF R is the critical receptor for BAFF during B lymphopoiesis. In contrast, BCMA- or TACI-deficient mice have no major defect in B cell development. While the function of BCMA is not defined, TACI has been shown to control B cell homeostasis and T cell-independent immune responses.

  1. Rolink, A.G. and F. Melcher (2002) Curr. Opin. Immunol. 14:266.
  2. Mackay F. and J.L. Browning (2002) Nature Reviews Immunology 2:464.
  3. Laabi, Y. et al. (2001) Current Biol. 11:R1013.
  4. Thompson, J.S. et al. (2001) Science 14:2108.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for BAFF R/TNFRSF13C Antibody (AF1162)(12)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 4 applications: Flow, ICC, IHC Paraffin-embedded, Neut.


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Flow
(6)
ICC
(1)
IHC Paraffin-embedded
(1)
Neut
(4)
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Human
(12)
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Showing Publications 1 - 10 of 12. Show All 12 Publications.
Publications using AF1162 Applications Species
Saulep-Easton D, Vincent F, Quah P, Wei A, Ting S, Croce C, Tam C, Mackay F The BAFF receptor TACI controls IL-10 production by regulatory B cells and CLL B cells. Leukemia, 2016;30(1):163-72. 2016 [PMID: 26139429] (Neut,, Human) Neut Human
Maddur M, Sharma M, Hegde P, Stephen-Victor E, Pulendran B, Kaveri S, Bayry J Human B cells induce dendritic cell maturation and favour Th2 polarization by inducing OX-40 ligand. Nat Commun, 2014;5(0):4092. 2014 [PMID: 24910129] (Neut,, Human) Neut Human
Notas G, Alexaki V, Kampa M, Pelekanou V, Charalampopoulos I, Sabour-Alaoui S, Pediaditakis I, Dessirier V, Gravanis A, Stathopoulos E, Tsapis A, Castanas E APRIL Binding to BCMA Activates a JNK2-FOXO3-GADD45 Pathway and Induces a G2/M Cell Growth Arrest in Liver Cells. J Immunol, 2012;189(10):4748-58. 2012 [PMID: 23071284] (ICC,, Human) ICC Human
Luster, Troy A, Mukherjee, Ipsita, Carrell, Jeffrey, Cho, Yun Hee, Gill, Jeffrey, Kelly, Lizbeth, Garcia, Andy, Ward, Christop, Oh, Luke, Ullrich, Stephen, Migone, Thi-Sau, Humphreys, Robin Fusion Toxin BLyS-Gelonin Inhibits Growth of Malignant Human B Cell Lines In Vitro and In Vivo. PLoS ONE, 2012;7(10):e47361. 2012 [PMID: 23056634] (Neut,, Human) Neut Human
Ohmatsu H, Sugaya M, Miyagaki T, Suga H, Fujita H, Asano Y, Tada Y, Kadono T, Sato S BAFF levels are increased in lesional skin and sera in patients with cutaneous T-cell lymphoma. Br. J. Dermatol., 2012;167(2):359-67. 2012 [PMID: 22512719] (IHC Paraffin-embedded,, Human) IHC Paraffin-embedded Human
Yeramilli VA, Knight KL Requirement for BAFF and APRIL during B cell development in GALT. J. Immunol., 2010;184(10):5527-36. 2010 [PMID: 20400696] (Flow,, Human) Flow Human
Badr G, Borhis G, Lefevre EA, Chaoul N, Deshayes F, Dessirier V, Lapree G, Tsapis A, Richard Y BAFF enhances chemotaxis of primary human B cells: a particular synergy between BAFF and CXCL13 on memory B cells. Blood, 2008;111(5):2744-54. 2008 [PMID: 18172003] (Flow,, Human) Flow Human
Neri P, Kumar S, Fulciniti MT, Vallet S, Chhetri S, Mukherjee S, Tai Y, Chauhan D, Tassone P, Venuta S, Munshi NC, Hideshima T, Anderson KC, Raje N Neutralizing B-cell activating factor antibody improves survival and inhibits osteoclastogenesis in a severe combined immunodeficient human multiple myeloma model. Clin. Cancer Res., 2007;13(19):5903-9. 2007 [PMID: 17908986] (Flow,, Human) Flow Human
Ettinger R, Sims GP, Robbins R, Withers D, Fischer RT, Grammer AC, Kuchen S, Lipsky PE IL-21 and BAFF/BLyS synergize in stimulating plasma cell differentiation from a unique population of human splenic memory B cells. J. Immunol., 2007;178(5):2872-82. 2007 [PMID: 17312131] (Flow,, Human) Flow Human
Elsawa SF, Novak AJ, Grote DM, Ziesmer SC, Witzig TE, Kyle RA, Dillon SR, Harder B, Gross JA, Ansell SM B-lymphocyte stimulator (BLyS) stimulates immunoglobulin production and malignant B-cell growth in Waldenstrom macroglobulinemia. Blood, 2006;107(7):2882-8. 2006 [PMID: 16304043] (Flow,, Human) Flow Human
Show All 12 Publications.

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Bioinformatics

Gene Symbol TNFRSF13C
Entrez
Uniprot