Recombinant Human BAFF R/TNFRSF13C Fc Chimera (Catalog #1162‑BR) inhibits Recombinant Human BAFF/BLyS/TNFSF13B (Catalog # 2149‑BF) induced proliferation in mouse B cells in a dose-dependent manner (orange line). ...read more
Peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD19 Fluorescein‑conjugated Monoclonal Antibody (Catalog # FAB4867F) and either (A) Goat Anti-Human BAFF R/TNFRSF13C Antigen ...read more
Mouse myeloma cell line NS0-derived recombinant human BAFF R/TNFRSF13C Arg2-Ala71 Accession # Q96RJ3
Detects human BAFF R in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross-reactivity with recombinant mouse BAFF R and less than 2% cross-reactivity with recombinant human (rh) 4-1BB, rhCD27, rhCD30, rhCD40, rhDR3, rhDR6, rhEDAR, rhFas, rhGITR, rhHVEM, rhNGF R, rhOPG, rhRANK, rhTNF RI, and rhTNF RII is observed.
<0.10 EU per 1 μg of the antibody by the LAL method.
Test in a species/application not listed above to receive a full credit towards a future purchase.
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Reconstitute at 0.2 mg/mL in sterile PBS.
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for BAFF R/TNFRSF13C Antibody [Unconjugated]
B cell activating factor (BAFF), also known as BlyS, TALL-1, TNAK, and zTNF4, is a TNF ligand superfamily member and has been designated TNFSF13B. Produced by macrophages, dendritic cells, and T lymphocytes, BAFF promotes the survival of B cells and is essential for B cell maturation (1-4). BAFF binds to three TNF receptor superfamily members: B cell maturation antigen (BCMA/TNFRSF17), transmembrane activator and calcium-modulator and cyclophilin ligand interactor (TACI/TNFRSF13B) and BAFF receptor (BAFF R/BR3/TNFRSF13C). These receptors are type III transmembrane proteins that lack a signal peptide. Whereas TACI and BCMA bind BAFF and another TNF superfamily ligand, APRIL (a proliferation-inducing ligand), BAFF R selectively binds BAFF. The BAFF R extracellular domain lacks the TNF receptor canonical cysteine-rich domain (CRD) and contains only a partial CRD with four cysteine residues. Human and mouse BAFF R share 56% aa sequence identity. BAFF R is highly expressed in spleen, lymph node and resting B cells. It is also expressed at lower levels in activated B cell, in resting CD4 + T cells, in thymus and peripheral blood leukocytes. BAFF knockout mice lack mature B cells. Similarly, A/WySnJ mice that are defective in BAFF-R intracellular signaling also lack mature B cells, suggesting that BAFF R is the critical receptor for BAFF during B lymphopoiesis. In contrast, BCMA- or TACI-deficient mice have no major defect in B cell development. While the function of BCMA is not defined, TACI has been shown to control B cell homeostasis and T cell-independent immune responses.
Rolink, A.G. and F. Melcher (2002) Curr. Opin. Immunol. 14:266.
Mackay F. and J.L. Browning (2002) Nature Reviews Immunology 2:464.
Laabi, Y. et al. (2001) Current Biol. 11:R1013.
Thompson, J.S. et al. (2001) Science 14:2108.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Publications for BAFF R/TNFRSF13C Antibody (AF1162)(12)
We have publications tested in 1 confirmed species: Human.
We have publications tested in 4 applications: Flow, ICC, IHC Paraffin-embedded, Neut.
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